Cofilin antibody (AA 3-98)

Catalog No. ABIN968755

This Mouse Monoclonal antibody specifically detects Cofilin in WB and IF. It exhibits reactivity toward Human, Mouse, Rat and Dog and has been mentioned in 3+ publications.

Quick Overview for Cofilin antibody (AA 3-98) (ABIN968755)

Target: Cofilin (CFL1) (Cofilin 1 (CFL1))

Reactivity: Human, Mouse, Rat, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cofilin antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: 32-Cofilin

Product Details anti-Cofilin Antibody

Binding Specificity: AA 3-98

Cross-Reactivity: Human, Dog (Canine), Rat (Rattus)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse Cofilin aa. 3-98

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN968540, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

References for anti-Cofilin antibody (ABIN968755)

Chan, Bailly, Zebda, Segall, Condeelis: "Role of cofilin in epidermal growth factor-stimulated actin polymerization and lamellipod protrusion." in: The Journal of cell biology, Vol. 148, Issue 3, pp. 531-42, (2000) (PubMed).

Moriyama, Yahara: "Two activities of cofilin, severing and accelerating directional depolymerization of actin filaments, are affected differentially by mutations around the actin-binding helix." in: The EMBO journal, Vol. 18, Issue 23, pp. 6752-61, (2000) (PubMed).

Moriyama, Matsumoto, Nishida, Sakai, Yahara: "Nucleotide sequence of mouse cofilin cDNA." in: Nucleic acids research, Vol. 18, Issue 10, pp. 3053, (1990) (PubMed).

Target Details for Cofilin

Target: Cofilin (CFL1) (Cofilin 1 (CFL1))

Alternative Name: Cofilin

Background: Cell motility is a basic cellular behavior involved in embryogenesis, neurite outgrowth, wound healing, inflammation, and cancer metastasis. Filamentous actin is an essential component of cell protrusions involved in cell motility. The protrusion formation is regulated by actin polymerization and depolymerization. Cofilin is a ubiquitously expressed G- and F-actin binding protein that contains a nuclear localization signal, a C-terminal hexapeptide sequence that is identical to tropomyosin, and other regions that are homologous to actin binding proteins. In vitro, cofilin has actin severing activity and induces an increased off-rate from the pointed end of actin filaments. These activities can increase the rate of actin polymerization and depolymerization, as well as increase the number of barbed ends available for polymerization. Stimulation of MTLn3 cells with EGF leads to an increase in cofilin at the leading edge of laemellipodia, which correlates with an increase in the number of barbed ends at the leading edge. Cofilin function blocking antibodies inhibit both the appearance of these barbed ends and lamellipodial protrusion. Thus, cofilin is an actin binding protein that regulates actin filament formation during cell motility.

Molecular Weight: 19 kDa

Pathways: Regulation of Cell Size, Tube Formation, Protein targeting to Nucleus, CXCR4-mediated Signaling Events