beta Actin antibody (N-Term)

Catalog No. ABIN968977

This Mouse Monoclonal antibody specifically detects beta Actin in WB, ELISA, ICC and FACS. It exhibits reactivity toward Human, Mouse, Rat, Dog, Monkey and Hamster and has been mentioned in 5+ publications.

Quick Overview for beta Actin antibody (N-Term) (ABIN968977)

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Reactivity: Human, Mouse, Rat, Dog, Monkey, Hamster

Host: Mouse

Clonality: Monoclonal

Conjugate: This beta Actin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Flow Cytometry (FACS)

Clone: 8H10D10

Product Details anti-beta Actin Antibody

Binding Specificity: N-Term

Purpose: beta-Actin Antibody

Purification: Ascitic fluid

Immunogen: Synthetic peptide corresponding to amino-terminal residues of human beta-Actin, conjugated to KLH.

Isotype: IgG2b

Application Details

Application Notes:

ELISA: 1/10000

FCM: 1/200 - 1/400

ICC: 1/200 - 1/1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Ascitic fluid containing 0.03 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

References for anti-beta Actin antibody (ABIN968977)

Zou, Ren, Liu, Fu, Chen, Li, Luo, He, Gao, Zeng, Xiong, Li, Huang, Xu, Zhang: "Inhibin B suppresses anoikis resistance and migration through the transforming growth factor-β signaling pathway in nasopharyngeal carcinoma." in: Cancer science, Vol. 109, Issue 11, pp. 3416-3427, (2018) (PubMed).

Rual, Venkatesan, Hao, Hirozane-Kishikawa, Dricot, Li, Berriz, Gibbons, Dreze, Ayivi-Guedehoussou, Klitgord, Simon, Boxem, Milstein, Rosenberg, Goldberg, Zhang, Wong, Franklin, Li, Albala, Lim et al.: "Towards a proteome-scale map of the human protein-protein interaction network. ..." in: Nature, Vol. 437, Issue 7062, pp. 1173-8, (2005) (PubMed).

Xu, Wratten, Charych, Buyske, Firestein, Brzustowicz: "Increased expression in dorsolateral prefrontal cortex of CAPON in schizophrenia and bipolar disorder." in: PLoS medicine, Vol. 2, Issue 10, pp. e263, (2005) (PubMed).

Pederson, Aebi: "Nuclear actin extends, with no contraction in sight." in: Molecular biology of the cell, Vol. 16, Issue 11, pp. 5055-60, (2005) (PubMed).

Bruneel, Labas, Mailloux, Sharma, Royer, Vinh, Pernet, Vaubourdolle, Baudin: "Proteomics of human umbilical vein endothelial cells applied to etoposide-induced apoptosis." in: Proteomics, Vol. 5, Issue 15, pp. 3876-84, (2005) (PubMed).

Target Details for beta Actin

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Alternative Name: beta-Actin

Background: Beta-actin (PS1TP5-binding protein 1), also known as ACTB, PS1TP5BP1. Entrez Protein NP_001092. It is one of six different actin proteins. Actin, a ubiquitous eukaryotic protein, is the major component of the cytoskeleton.Actins are highly conserved proteins that are involved in various types of cell motility, structure, and integrity. Actin is ubiquitously expressed in all eukaryotic cells. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.

Molecular Weight: 42 kDa

UniProt: P60709

Pathways: Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction