Cyclin B1 antibody

Catalog No. ABIN969006

This anti-Cyclin B1 antibody is a Mouse Monoclonal antibody detecting Cyclin B1 in WB, ELISA, FACS and ICC. Suitable for Human and Rat. This Primary Antibody has been cited in 2+ publications.

Quick Overview for Cyclin B1 antibody (ABIN969006)

Target: Cyclin B1 (CCNB1)

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cyclin B1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC)

Clone: 5G6

Product Details anti-Cyclin B1 Antibody

Purpose: CCNB1 Antibody

Purification: Ascitic fluid

Immunogen: Purified recombinant fragment of human CCNB1 expressed in E. Coli.

Isotype: IgG1

Application Details

Application Notes:

ELISA: 1/10000

FCM: 1/200 - 1/400

ICC: 1/200 - 1/1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Ascitic fluid containing 0.03 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

References for anti-Cyclin B1 antibody (ABIN969006)

Chou, Yen, Lee, Chen: "Pro-apoptotic role of Cdc25A: activation of cyclin B1/Cdc2 by the Cdc25A C-terminal domain." in: The Journal of biological chemistry, Vol. 285, Issue 23, pp. 17833-45, (2010) (PubMed).

Gustafsson, Zhao, Gustafsson, Dahlman-Wright: "RBCK1 drives breast cancer cell proliferation by promoting transcription of estrogen receptor alpha and cyclin B1." in: Cancer research, Vol. 70, Issue 3, pp. 1265-74, (2010) (PubMed).

Target Details for Cyclin B1

Target: Cyclin B1 (CCNB1)

Alternative Name: CCNB1

Background: The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. (provided by RefSeq) It has higher expression in tumor tissues .

Molecular Weight: 60 kDa

Gene ID: 891

UniProt: P14635

Pathways: Cell Division Cycle, AMPK Signaling, Mitotic G1-G1/S Phases, M Phase