CCT2 antibody

Catalog No. ABIN969010

This Mouse Monoclonal antibody specifically detects CCT2 in WB, ELISA, ICC and FACS. It exhibits reactivity toward Human, Mouse, Rat and Monkey and has been mentioned in 2+ publications.

Quick Overview for CCT2 antibody (ABIN969010)

Target: CCT2 (Chaperonin Containing TCP1, Subunit 2 (Beta) (CCT2))

Reactivity: Human, Mouse, Rat, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This CCT2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Flow Cytometry (FACS)

Clone: 5B5C4

Product Details anti-CCT2 Antibody

Purpose: CCT2 Antibody

Purification: Ascitic fluid

Immunogen: Purified recombinant fragment of human CCT2 expressed in E. Coli.

Isotype: IgG1

Application Details

Application Notes:

ELISA: 1/10000

FCM: 1/200 - 1/400

ICC: 1/200 - 1/1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Ascitic fluid containing 0.03 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

References for anti-CCT2 antibody (ABIN969010)

Abe, Yoon, Kubota, Mendoza, Gygi, Blenis: "p90 ribosomal S6 kinase and p70 ribosomal S6 kinase link phosphorylation of the eukaryotic chaperonin containing TCP-1 to growth factor, insulin, and nutrient signaling." in: The Journal of biological chemistry, Vol. 284, Issue 22, pp. 14939-48, (2009) (PubMed).

Goudreault, DAmbrosio, Kean, Mullin, Larsen, Sanchez, Chaudhry, Chen, Sicheri, Nesvizhskii, Aebersold, Raught, Gingras: "A PP2A phosphatase high density interaction network identifies a novel striatin-interacting phosphatase and kinase complex linked to the cerebral cavernous malformation 3 (CCM3) protein." in: Molecular & cellular proteomics : MCP, Vol. 8, Issue 1, pp. 157-71, (2009) (PubMed).

Target Details for CCT2

Target: CCT2 (Chaperonin Containing TCP1, Subunit 2 (Beta) (CCT2))

Alternative Name: CCT2

Background: The protein encoded by this gene is a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Two transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 54 kDa

UniProt: P78371