CD44 antibody

Catalog No. ABIN969026

The Mouse Monoclonal anti-CD44 antibody has been validated for FACS, IHC, ELISA and ICC. It is suitable to detect CD44 in samples from Human and Mouse. There are 5+ publications available.

Quick Overview for CD44 antibody (ABIN969026)

Target: CD44

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD44 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC)

Clone: 8E2F3

Product Details anti-CD44 Antibody

Purpose: CD44 Antibody

Purification: Ascitic fluid

Immunogen: Purified recombinant fragment of human CD44 (628-699) expressed in E. Coli.

Isotype: IgG1

Application Details

Application Notes:

ELISA: 1/10000

FCM: 1/200 - 1/400

ICC: 1/200 - 1/1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Ascitic fluid containing 0.03 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

References for anti-CD44 antibody (ABIN969026)

Bychkov, Kirichenko, Mikhaylova, Paramonov, Yastremsky, Kirpichnikov, Shulepko, Lyukmanova: "Extracellular Vesicles Derived from Acidified Metastatic Melanoma Cells Stimulate Growth, Migration, and Stemness of Normal Keratinocytes." in: , Vol. 10, Issue 3, (2022) (PubMed).

Bychkov, Kirichenko, Shulepko, Mikhaylova, Kirpichnikov, Lyukmanova: "Mambalgin-2 Inhibits Growth, Migration, and Invasion of Metastatic Melanoma Cells by Targeting the Channels Containing an ASIC1a Subunit Whose Up-Regulation Correlates with Poor Survival Prognosis." in: Biomedicines, Vol. 9, Issue 10, (2021) (PubMed).

Klein: "Improved Isolation of Human Vascular Wall-Resident Mesenchymal Stem Cells." in: Methods in molecular biology (Clifton, N.J.), Vol. 2155, pp. 71-81, (2020) (PubMed).

Barbe, Lundberg, Oksvold, Stenius, Lewin, Björling, Asplund, Pontén, Brismar, Uhlén, Andersson-Svahn: "Toward a confocal subcellular atlas of the human proteome." in: Molecular & cellular proteomics : MCP, Vol. 7, Issue 3, pp. 499-508, (2008) (PubMed).

Subramaniam, Gardner, Jothy: "Soluble CD44 secretion contributes to the acquisition of aggressive tumor phenotype in human colon cancer cells." in: Experimental and molecular pathology, Vol. 83, Issue 3, pp. 341-6, (2007) (PubMed).

Target Details for CD44

Target: CD44

Alternative Name: CD44

Background: CD44, also known as IN, LHRMIC4, CDW44, HCELL. It is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis.

Molecular Weight: 82 kDa

UniProt: P16070

Pathways: Glycosaminoglycan Metabolic Process, Autophagy, Negative Regulation of intrinsic apoptotic Signaling