N-Cadherin antibody

Catalog No. ABIN969038

The Mouse Monoclonal anti-N-Cadherin antibody has been validated for WB, IHC, FACS, ELISA and ICC. It is suitable to detect N-Cadherin in samples from Human, Mouse and Rat. There are 2+ publications available.

Quick Overview for N-Cadherin antibody (ABIN969038)

Target: N-Cadherin (CDH2) (Cadherin 2 (CDH2))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This N-Cadherin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), ELISA, Immunocytochemistry (ICC)

Clone: 5D5

Product Details anti-N-Cadherin Antibody

Purpose: CDH2 Antibody

Purification: Ascitic fluid

Immunogen: Purified recombinant fragment of human CDH2 expressed in E. Coli.

Isotype: IgG1

Application Details

Application Notes:

ELISA: 1/10000

FCM: 1/200 - 1/400

ICC: 1/200 - 1/1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Ascitic fluid containing 0.03 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

References for anti-N-Cadherin antibody (ABIN969038)

Wallerand, Cai, Wainberg, Garraway, Lascombe, Nicolle, Thiery, Bittard, Radvanyi, Reiter: "Phospho-Akt pathway activation and inhibition depends on N-cadherin or phospho-EGFR expression in invasive human bladder cancer cell lines." in: Urologic oncology, Vol. 28, Issue 2, pp. 180-8, (2010) (PubMed).

Sharma, Henderson: "IQ-domain GTPase-activating protein 1 regulates beta-catenin at membrane ruffles and its role in macropinocytosis of N-cadherin and adenomatous polyposis coli." in: The Journal of biological chemistry, Vol. 282, Issue 11, pp. 8545-56, (2007) (PubMed).

Target Details for N-Cadherin

Target: N-Cadherin (CDH2) (Cadherin 2 (CDH2))

Alternative Name: CDH2

Background: This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry. At certain central nervous system synapses, presynaptic to postsynaptic adhesion is mediated at least in part by this gene product.

Molecular Weight: 99.8 kDa

UniProt: P19022

Pathways: Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Synaptic Membrane