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KRT8 antibody (AA 391-483)

Name: KRT8 antibody (AA 391-483)
Brand: antibodies-online
SKU: ABIN969080
Price: 548.9 USD
Availability: InStock

The Mouse Monoclonal anti-KRT8 antibody has been validated for WB, IHC, ELISA and ICC. It is suitable to detect KRT8 in samples from Human. There is 1 publication available.

Western blot analysis using CK8 mouse mAb against A549 (1), Hela (2), MCF-7 (3), A431 (4) and HepG2 (5) cell lysate. (KRT8 antibody (AA 391-483))

Confocal immunofluorescence analysis of methanol-fixed ECA109 cells (left) and HepG2 cells (right) using CK8 mouse mAb (green), showing cytoplasmic localization. (KRT8 antibody (AA 391-483))

Immunohistochemical analysis of paraffin-embedded human Kidney tissues using CK8 mouse mAb (KRT8 antibody (AA 391-483))

IHC

Immunohistochemical analysis of paraffin-embedded human breast carcinoma (A), lung cancer (B) and ovarian cancer tissue (C), showing membrane and cytoplasmic localization with DAB staining using CK8 mouse mAb. (KRT8 antibody (AA 391-483))

IHC

4 images

(1 reference)

Catalog No. ABIN969080

Quick Overview for KRT8 antibody (AA 391-483) (ABIN969080)

Target

See all KRT8 Antibodies

KRT8 (Keratin 8 (KRT8))

Reactivity

Human

Host

Mouse

Clonality

Monoclonal

Conjugate

This KRT8 antibody is un-conjugated

Application

Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC)

Clone

8A5D12

Discover our top product KRT8 Primary Antibody

Binding Specificity
23

16

13

11

10

8

6

6

5

5

5

4

4

3

3

3

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 391-483

Purpose

Cytokeratin 8 Antibody

Purification

Ascitic fluid

Immunogen

Purified recombinant fragment of human Cytokeratin (aa391-483) expressed in E. Coli.

Isotype

IgG1
anti-Keratin 8 (KRT8) antibody
KRT8 Reactivity: Human, Mouse WB, IHC, FACS, ICC, IHC (fro) Host: Mouse Monoclonal M20 unconjugated

anti-Keratin 8 (KRT8) (AA 92-393) antibody
KRT8 Reactivity: Human WB, IHC, IF, FACS, ICC Host: Rabbit Polyclonal unconjugated

anti-Keratin 8 (KRT8) (AA 1-483) antibody
KRT8 Reactivity: Human WB, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-Keratin 8 (KRT8) (Ser726), (Ser731) antibody
KRT8 Reactivity: Human WB, ELISA, IHC (p) Host: Rabbit Polyclonal unconjugated

anti-Keratin 8 (KRT8) (pSer432) antibody
KRT8 Reactivity: Human, Rat, Mouse WB, IHC, IF, ELISA, ICC Host: Rabbit Polyclonal unconjugated

anti-Keratin 8 (KRT8) antibody
KRT8 Reactivity: Human, Rat, Zebrafish (Danio rerio) WB, IHC, IF, FACS, StM Host: Mouse Monoclonal H1 unconjugated

anti-Keratin 8 (KRT8) (AA 91-381) antibody
KRT8 Reactivity: Human, Rat, Monkey, Dog WB, IHC, IF, IHC (p) Host: Mouse Monoclonal 1B12 unconjugated

anti-Keratin 8 (KRT8) antibody
KRT8 Reactivity: Human, Cow, Rabbit, Pig, Sheep IHC, IF, FACS, StM Host: Mouse Monoclonal B22-1 unconjugated

anti-Keratin 8 (KRT8) (Middle Region) antibody
KRT8 Reactivity: Human, Rat, Mouse, Cow, Rabbit, Pig, Dog, Guinea Pig, Horse WB, IHC Host: Rabbit Polyclonal unconjugated

anti-Keratin 8 (KRT8) (N-Term) antibody
KRT8 Reactivity: Human, Rat, Mouse, Cow, Dog, Guinea Pig, Horse, Saccharomyces cerevisiae WB, IHC Host: Rabbit Polyclonal unconjugated

Application Notes

ELISA: 1/10000
ICC: 1/200 - 1/1000

Restrictions

For Research Use only
Format

Liquid

Buffer

Ascitic fluid containing 0.03 % sodium azide.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Walker, Harris, Holloway, McKenzie, Wells, Robinson, Morris: "Cytokeratin KRT8/18 expression differentiates distinct subtypes of grade 3 invasive ductal carcinoma of the breast." in: Cancer genetics and cytogenetics, Vol. 178, Issue 2, pp. 94-103, (2007) (PubMed).
Target

KRT8 (Keratin 8 (KRT8))

Alternative Name

Cytokeratin 8

Background

Cytokeratin 8, also known as CK8, KRT8, K8. Entrez Protein NP_002264. It is a member of the type II keratin family.Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells.It typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis.

Molecular Weight

54 kDa

Gene ID

3856

UniProt

P05787