DLK1 antibody

Catalog No. ABIN969086

The Mouse Monoclonal anti-DLK1 antibody has been validated for ELISA, FACS and ICC. It is suitable to detect DLK1 in samples from Human. There are 2+ publications available.

Quick Overview for DLK1 antibody (ABIN969086)

Target: DLK1 (delta-Like 1 Homolog (Drosophila) (DLK1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This DLK1 antibody is un-conjugated

Application: ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC)

Clone: 3A10

Product Details anti-DLK1 Antibody

Purpose: DLK1 Antibody

Purification: Ascitic fluid

Immunogen: Purified recombinant fragment of human DLK1 expressed in E. Coli.

Isotype: IgG1

Application Details

Application Notes:

ELISA: 1/10000

FCM: 1/200 - 1/400

ICC: 1/200 - 1/1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Ascitic fluid containing 0.03 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

References for anti-DLK1 antibody (ABIN969086)

Espina, Méndez-Vidal, Moreno-Mateos, Sáez, Romero-Franco, Japón, Pintor-Toro: "Induction of Dlk1 by PTTG1 inhibits adipocyte differentiation and correlates with malignant transformation." in: Molecular biology of the cell, Vol. 20, Issue 14, pp. 3353-62, (2009) (PubMed).

Katrincsakova, Takeda, Urbankova, Michaux, Jarosova, Vandenberghe, Georges, Charlier, Wlodarska: "Methylation analysis of the imprinted DLK1-GTL2 domain supports the random parental origin of the IGH-involving del(14q) in B-cell malignancies." in: Epigenetics, Vol. 4, Issue 7, pp. 469-75, (2009) (PubMed).

Target Details for DLK1

Target: DLK1 (delta-Like 1 Homolog (Drosophila) (DLK1))

Alternative Name: DLK1

Background: This gene encodes a transmembrane protein containing six epidermal growth factor repeats. The protein is involved in the differentiation of several cell types, including adipocytes, it is also thought to be a tumor suppressor. It is one of several imprinted genes located in a region of on chr 14q32. Certain mutations in this imprinted region can cause phenotypes similar to maternal and paternal uniparental disomy of chromosome 14 (UPD14). This gene is expressed from the paternal allele. A polymorphism within this gene has been associated with child and adolescent obesity. The mode of inheritance for this polymorphism is polar overdominance, this non-Mendelian inheritance pattern was first described in sheep with the callipyge phenotype, which is characterized by muscle hypertrophy and decreased fat mass.

Molecular Weight: 41 kDa

Gene ID: 8788

UniProt: P80370