GAPDH antibody

Catalog No. ABIN969164

The Mouse Monoclonal anti-GAPDH antibody has been validated for WB, ELISA, IHC and ICC. It is suitable to detect GAPDH in samples from Human, Mouse and Rat. There are 4+ publications available.

Quick Overview for GAPDH antibody (ABIN969164)

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This GAPDH antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Clone: 1A10

Product Details anti-GAPDH Antibody

Purpose: GAPDH Antibody

Purification: Purified antibody

Immunogen: Purified recombinant fragment of human GAPDH expressed in E. Coli.

Isotype: IgG1

Application Details

Application Notes:

ELISA: 1/10000

ICC: 1/200 - 1/1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified antibody in PBS with 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

References for anti-GAPDH antibody (ABIN969164)

Xu, Xu, Gin, Nshimiyimana, Mooers, Caputi, Hannafon, Ding: "SRSF1 regulates exosome microRNA enrichment in human cancer cells." in: Cell communication and signaling : CCS, Vol. 18, Issue 1, pp. 130, (2020) (PubMed).

Hannafon, Gin, Xu, Bruns, Calloway, Ding: "Metastasis-associated protein 1 (MTA1) is transferred by exosomes and contributes to the regulation of hypoxia and estrogen signaling in breast cancer cells." in: Cell communication and signaling : CCS, Vol. 17, Issue 1, pp. 13, (2019) (PubMed).

Wang, Hannafon, Wolf, Zhou, Avery, Wu, Lind, Ding: "Characterization of docosahexaenoic acid (DHA)-induced heme oxygenase-1 (HO-1) expression in human cancer cells: the importance of enhanced BTB and CNC homology 1 (Bach1) degradation." in: The Journal of nutritional biochemistry, Vol. 25, Issue 5, pp. 515-25, (2015) (PubMed).

Sumner, Huynh, Markowitz, Perhac, Hill, Coovert, Schussler, Chen, Jarecki, Burghes, Taylor, Fischbeck: "Valproic acid increases SMN levels in spinal muscular atrophy patient cells." in: Annals of neurology, Vol. 54, Issue 5, pp. 647-54, (2003) (PubMed).

Target Details for GAPDH

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Alternative Name: GAPDH

Background: Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. It catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Besides its functioning as a glycolytic enzyme in cytoplasm, recent evidence suggest that mammalian GAPDH is also involved in a great number of intracellular proceses such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication, and DNA repair. During the last decade a lot of findings appeared concerning the role of GAPDH in different pathologies including prostate cancer progression, programmed neuronal cell death, age- related neuronal diseases, such as Alzheimer

Molecular Weight: 37 kDa

Gene ID: 2597

UniProt: P04406