GRK2 antibody

Catalog No. ABIN969178

Product Details anti-GRK2 Antibody

Target: GRK2 (ADRBK1) (Adrenergic, Beta, Receptor Kinase 1 (ADRBK1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This GRK2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Purpose: GRK2 Antibody

Purification: Ascitic fluid

Immunogen: Purified recombinant fragment of human GRK2 expressed in E. Coli.

Clone: 3F8

Isotype: IgG1

Application Details

Application Notes:

ELISA: 1/10000

ICC: 1/200 - 1/1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Ascitic fluid containing 0.03 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

References for anti-GRK2 antibody (ABIN969178)

Sterne-Marr, Leahey, Bresee, Dickson, Ho, Ragusa, Donnelly, Amie, Krywy, Brookins-Danz, Orakwue, Carr, Yoshino-Koh, Li, Tesmer: "GRK2 activation by receptors: role of the kinase large lobe and carboxyl-terminal tail." in: Biochemistry, Vol. 48, Issue 20, pp. 4285-93, (2009) (PubMed).

Chen, Long, Wu, Jiang, Ma: "EGF transregulates opioid receptors through EGFR-mediated GRK2 phosphorylation and activation." in: Molecular biology of the cell, Vol. 19, Issue 7, pp. 2973-83, (2008) (PubMed).

Target Details for GRK2

Target: GRK2 (ADRBK1) (Adrenergic, Beta, Receptor Kinase 1 (ADRBK1))

Alternative Name: GRK2 (ADRBK1 Products)

Background:

Description: The product of this gene phosphorylates the beta-2-adrenergic receptor and appears to mediate agonist-specific desensitization observed at high agonist concentrations. This protein is an ubiquitous cytosolic enzyme that specifically phosphorylates the activated form of the beta-adrenergic and related G-protein-coupled receptors. Abnormal coupling of beta-adrenergic receptor to G protein is involved in the pathogenesis of the failing heart. (provided by RefSeq)Tissue specificity: Expressed in peripheral blood leukocytes

Aliases: GRK2, BARK1, FLJ16718, BETA-ARK1, ADRBK1

Molecular Weight: 80kDa

Gene ID: 156

HGNC: 156

UniProt: P25098

Pathways: EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of G-Protein Coupled Receptor Protein Signaling, CXCR4-mediated Signaling Events, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling