HPRT1 antibody

Catalog No. ABIN969197

The Mouse Monoclonal anti-HPRT1 antibody has been validated for ELISA. It is suitable to detect HPRT1 in samples from Human. There is 1 publication available.

Quick Overview for HPRT1 antibody (ABIN969197)

Target: HPRT1 (Hypoxanthine phosphoribosyltransferase 1 (HPRT1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This HPRT1 antibody is un-conjugated

Application: ELISA

Clone: 1F8D11

Product Details anti-HPRT1 Antibody

Purpose: HPRT Antibody

Purification: Ascitic fluid

Immunogen: Purified recombinant fragment of HPRT expressed in E. Coli.

Isotype: IgG2b

Application Details

Application Notes: ELISA: 1/10000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Ascitic fluid containing 0.03 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

References for anti-HPRT1 antibody (ABIN969197)

Manjanatha, Shelton, Bishop, Shaddock, Dobrovolsky, Heflich, Webb, Blankenship, Beland, Greenlees, Culp: "Analysis of mutations and bone marrow micronuclei in Big Blue rats fed leucomalachite green." in: Mutation research, Vol. 547, Issue 1-2, pp. 5-18, (2004) (PubMed).

Target Details for HPRT1

Target: HPRT1 (Hypoxanthine phosphoribosyltransferase 1 (HPRT1))

Alternative Name: HPRT

Background: The HPRT1 gene provides instructions for making an enzyme called hypoxanthine phosphoribosyltransferase 1. This enzyme allows cells to recycle purines, some of the building blocks of DNA and its chemical cousin RNA.The enzyme hypoxanthine-guanine phosphoribosyltrasferase (E.C.2.4.2.8., HPRT) plays a crucial role in uric acid synthesis and purine metabolism.This enzyme catalyzes the conversion of hypoxanthine and guanine to inosine monophosphate (IMP) and guanosine monophosphate (GMP), respectively, and uses phosphoribosylpyrophosphate (PRPP) as a cosubstrate and as a source of energy . This pathway is also known as the purine salvage pathway because it allows cells to reuse purine compounds to build DNA and RNA.

UniProt: P00492

Pathways: Ribonucleoside Biosynthetic Process