KLHL13 antibody

Catalog No. ABIN969245

The Mouse Monoclonal anti-KLHL13 antibody has been validated for WB, ELISA, IHC, FACS and ICC. It is suitable to detect KLHL13 in samples from Human. There are 2+ publications available.

Quick Overview for KLHL13 antibody (ABIN969245)

Target: KLHL13 (Kelch-Like 13 (KLHL13))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This KLHL13 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Clone: 8D1

Product Details anti-KLHL13 Antibody

Purpose: KLHL13 Antibody

Purification: Ascitic fluid

Immunogen: Purified recombinant fragment of human KLHL13 expressed in E. Coli.

Isotype: IgG1

Application Details

Application Notes:

ELISA: 1/10000

FCM: 1/200 - 1/400

ICC: 1/200 - 1/1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Ascitic fluid containing 0.03 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

References for anti-KLHL13 antibody (ABIN969245)

Jin, Pahuja, Wickliffe, Gorur, Baumgärtel, Schekman, Rape: "Ubiquitin-dependent regulation of COPII coat size and function." in: Nature, Vol. 482, Issue 7386, pp. 495-500, (2012) (PubMed).

Suzuki, Yamashita, Shirota, Sakakibara, Chiba, Mizushima-Sugano, Nakai, Sugano: "Sequence comparison of human and mouse genes reveals a homologous block structure in the promoter regions." in: Genome research, Vol. 14, Issue 9, pp. 1711-8, (2004) (PubMed).

Target Details for KLHL13

Target: KLHL13 (Kelch-Like 13 (KLHL13))

Alternative Name: KLHL13

Background: KLHL13 (kelch-like 13), also known as BKLHD2 (BTB and kelch domain-containing protein 2), is a 604 amino acid protein that contains six Kelch repeats and one BTB/POZ domain. Expressed predominantly in brain, KLHL13 is believed to play a role in protein ubiquitination and may function as a substrate- specific adapter of an E3 ubiquitin-protein ligase complex. E3 ligases accept a ubiquitin residue from an E2 ubiquitin-conjugating enzyme and immediately transfer that residue to a protein that is targeted for degradation. Specifically, KLHL13 interacts with KLHL9 and CUL-3, a member of the cullin family of mediators that participate in the selective targeting of proteins for ubiquitin-mediated proteolysis. Via its BTB and C-terminal Kelch (BACK) motif, KLHL13 is thought to play a role in spatially orientating substrates in the CUL-3 ligase.

Molecular Weight: 74 kDa

UniProt: Q9P2N7