OCT4 antibody

Catalog No. ABIN969329

The Mouse Monoclonal anti-OCT4 antibody has been validated for WB, ELISA, ICC and FACS. It is suitable to detect OCT4 in samples from Human. There are 3+ publications available.

Quick Overview for OCT4 antibody (ABIN969329)

Target: OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This OCT4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Flow Cytometry (FACS)

Clone: 7E7

Product Details anti-OCT4 Antibody

Purpose: 4-Oct Antibody

Purification: Ascitic fluid

Immunogen: Synthesized peptide derived from internal of human Oct4.

Isotype: IgG1

Application Details

Application Notes:

ELISA: 1/10000

FCM: 1/200 - 1/400

ICC: 1/200 - 1/1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Ascitic fluid containing 0.03 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

References for anti-OCT4 antibody (ABIN969329)

Wang, Dai: "Concise review: isoforms of OCT4 contribute to the confusing diversity in stem cell biology." in: Stem cells (Dayton, Ohio), Vol. 28, Issue 5, pp. 885-93, (2010) (PubMed).

Stefanovic, Pucéat: "[The dual role of OCT4]." in: Médecine sciences : M/S, Vol. 26, Issue 4, pp. 411-6, (2010) (PubMed).

Rose, Behm, Drgon, Johnson, Uhl: "Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score." in: Molecular medicine (Cambridge, Mass.), Vol. 16, Issue 7-8, pp. 247-53, (2010) (PubMed).

Target Details for OCT4

Target: OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

Alternative Name: 4-Oct

Background: This gene encodes a transcription factor containing a POU homeodomain. This transcription factor plays a role in embryonic development, especially during early embryogenesis, and it is necessary for embryonic stem cell pluripotency. A translocation of this gene with the Ewing's sarcoma gene, t(6,22)(p21,q12), has been linked to tumor formation. Alternative splicing, as well as usage of alternative translation initiation codons, results in multiple isoforms, one of which initiates at a non-AUG (CUG) start codon. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12. (provided by RefSeq).Tissue specificity: Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues.

Molecular Weight: 45 kDa

UniProt: Q01860

Pathways: Stem Cell Maintenance