PRKDC antibody

Catalog No. ABIN969366

Product Details anti-PRKDC Antibody

Target: PRKDC (Protein Kinase, DNA-Activated, Catalytic Polypeptide (PRKDC))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PRKDC antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Purpose: PRKDC Antibody

Purification: Ascitic fluid

Immunogen: Purified recombinant fragment of human PRKDC expressed in E. Coli.

Clone: 3H6

Isotype: IgG1

Application Details

Application Notes:

ELISA: 1/10000

ICC: 1/200 - 1/1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Ascitic fluid containing 0.03 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

References for anti-PRKDC antibody (ABIN969366)

Durkin, Guo, Fryrear, Mihaylova, Gupta, Belgnaoui, Haoudi, Kupfer, Semmes: "HTLV-1 Tax oncoprotein subverts the cellular DNA damage response via binding to DNA-dependent protein kinase." in: The Journal of biological chemistry, Vol. 283, Issue 52, pp. 36311-20, (2008) (PubMed).

Huston, Lynch, Mohamed, Collins, Hill, MacLeod, Krause, Baillie, Houslay: "EPAC and PKA allow cAMP dual control over DNA-PK nuclear translocation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 35, pp. 12791-6, (2008) (PubMed).

Target Details for PRKDC

Target: PRKDC (Protein Kinase, DNA-Activated, Catalytic Polypeptide (PRKDC))

Alternative Name: PRKDC (PRKDC Products)

Background: The PRKDC gene encodes the catalytic subunit of a nuclear DNA-dependent serine/threonine protein kinase (DNA-PK). The second component is the autoimmune antigen Ku (MIM 152690), which is encoded by the G22P1 gene on chromosome 22q. On its own, the catalytic subunit of DNA-PK is inactive and relies on the G22P1 component to direct it to the DNA and trigger its kinase activity, PRKDC must be bound to DNA to express its catalytic properties.

Molecular Weight: 46.9 kDa

Gene ID: 5591

UniProt: P78527

Pathways: DNA Damage Repair, Production of Molecular Mediator of Immune Response