RAB25 antibody

Catalog No. ABIN969377

The Mouse Monoclonal anti-RAB25 antibody has been validated for ELISA, IHC, WB, FACS and ICC. It is suitable to detect RAB25 in samples from Human and Mouse. There is 1 publication available.

Quick Overview for RAB25 antibody (ABIN969377)

Target: RAB25 (RAB25, Member RAS Oncogene Family (RAB25))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This RAB25 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Clone: 3F12

Product Details anti-RAB25 Antibody

Purpose: Rab25 Antibody

Purification: Ascitic fluid

Immunogen: Purified recombinant fragment of Rab25 expressed in E. Coli.

Isotype: IgG1

Application Details

Application Notes:

ELISA: 1/10000

FCM: 1/200 - 1/400

ICC: 1/200 - 1/1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Ascitic fluid containing 0.03 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

References for anti-RAB25 antibody (ABIN969377)

Wang, Kumar, Navarre, Casanova, Goldenring: "Regulation of vesicle trafficking in madin-darby canine kidney cells by Rab11a and Rab25." in: The Journal of biological chemistry, Vol. 275, Issue 37, pp. 29138-46, (2000) (PubMed).

Target Details for RAB25

Target: RAB25 (RAB25, Member RAS Oncogene Family (RAB25))

Alternative Name: Rab25

Background: Members of the Ras-related superfamily of GTP binding proteins, which includes Ras, Rho, Rab and ARF subfamilies, exhibit 30-50 % similarity with Ras p21. Rab proteins play an important role for either in endocytosis or in biosynthetic protein transport. The possibility that Rab proteins might also direct the exocytosis from secretory vesicles to the plasma membrane is supported by the observation that in yeast, the SEC4 protein, which is 40 % similar to Rab proteins, is associated with secretory vesicles. Rab proteins located on the cytoplasmic face of organelles and vesicles, rab proteins are involved in intracellular membrane fusion reactions. Rab25 was cloned from a gastric parietal cell cDNA library and is expressed in epithelial tissues such as the gastrointestinal mucosae, kidney, and lung, which encoded a protein of 28 kDa.

Molecular Weight: 24 kDa

Gene ID: 57111

UniProt: P57735