SKP2 antibody (AA 1-130)

Catalog No. ABIN969399

Product Details anti-SKP2 Antibody

Target: SKP2 (S-Phase Kinase-Associated Protein 2 (SKP2))

Binding Specificity: AA 1-130

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SKP2 antibody is un-conjugated

Application: ELISA

Purpose: SKP2 Antibody

Purification: Ascitic fluid

Immunogen: Purified recombinant fragment of SKP2 (aa1-130) expressed in E. Coli.

Clone: 6G9D10

Isotype: IgG1

Application Details

Application Notes: ELISA: 1/10000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Ascitic fluid containing 0.03 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

References for anti-SKP2 antibody (ABIN969399)

Fujita, Liu, Doihara, Date, Wan: "Dissection of the APCCdh1-Skp2 cascade in breast cancer." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 14, Issue 7, pp. 1966-75, (2008) (PubMed).

Sun, Cai, Yu, Meng, Cheng, Zhao, Sui, Zhang: "Knockdown of S-phase kinase-associated protein-2 expression in MCF-7 inhibits cell growth and enhances the cytotoxic effects of epirubicin." in: Acta biochimica et biophysica Sinica, Vol. 39, Issue 12, pp. 999-1007, (2007) (PubMed).

Target Details for SKP2

Target: SKP2 (S-Phase Kinase-Associated Protein 2 (SKP2))

Alternative Name: SKP2 (SKP2 Products)

Background: SKP2: S-phase kinase-associated protein 2 (p45). This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class, in addition to an F-box, this protein contains 10 tandem leucine-rich repeats. This protein is an essential element of the cyclin A-CDK2 S-phase kinase. It specifically recognizes phosphorylated cyclin-dependent kinase inhibitor 1B (CDKN1B, also referred to as p27 or KIP1) predominantly in S phase and interacts with S-phase kinase-associated protein 1 (SKP1 or p19). In addition, this gene is established as a protooncogene causally involved in the pathogenesis of lymphomas. Alternative splicing of this gene generates 2 transcript variants encoding different isoforms.

UniProt: Q13309

Pathways: Mitotic G1-G1/S Phases