SMAD4 antibody

Catalog No. ABIN969403

This Mouse Monoclonal antibody specifically detects SMAD4 in WB, ELISA, IHC, ICC and FACS. It exhibits reactivity toward Human and Mouse and has been mentioned in 2+ publications.

Quick Overview for SMAD4 antibody (ABIN969403)

Target: SMAD4 (SMAD Family Member 4 (SMAD4))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This SMAD4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Clone: 4G1C6

Product Details anti-SMAD4 Antibody

Purpose: SMAD4 Antibody

Purification: Ascitic fluid

Immunogen: Purified recombinant fragment of human SMAD4 expressed in E. Coli.

Isotype: IgG1

Application Details

Application Notes:

ELISA: 1/10000

FCM: 1/200 - 1/400

ICC: 1/200 - 1/1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Ascitic fluid containing 0.03 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

References for anti-SMAD4 antibody (ABIN969403)

Yao, Yin, Lian, Tian, Liu, Li, Sun: "MicroRNA-224 is involved in transforming growth factor-beta-mediated mouse granulosa cell proliferation and granulosa cell function by targeting Smad4." in: Molecular endocrinology (Baltimore, Md.), Vol. 24, Issue 3, pp. 540-51, (2010) (PubMed).

Talmud, Drenos, Shah, Shah, Palmen, Verzilli, Gaunt, Pallas, Lovering, Li, Casas, Sofat, Kumari, Rodriguez, Johnson, Newhouse, Dominiczak, Samani, Caulfield, Sever, Stanton, Shields, Padmanabhan et al.: "Gene-centric association signals for lipids and apolipoproteins identified via the HumanCVD BeadChip. ..." in: American journal of human genetics, Vol. 85, Issue 5, pp. 628-42, (2009) (PubMed).

Target Details for SMAD4

Target: SMAD4 (SMAD Family Member 4 (SMAD4))

Alternative Name: SMAD4

Background: Common mediator of signal transduction by TGF-beta (transforming growth factor) superfamily, SMAD4 is the common SMAD (co-SMAD). Promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. May act as a tumor suppressor Mutations or deletions in this gene have been shown to result in pancreatic cancer, juvenile polyposis syndrome, and hereditary hemorrhagic telangiectasia syndrome.

Molecular Weight: 65 kDa

UniProt: Q13485

Pathways: Cell Division Cycle, Chromatin Binding, Autophagy