XBP1 antibody

Catalog No. ABIN969462

This anti-XBP1 antibody is a Mouse Monoclonal antibody detecting XBP1 in ELISA. Suitable for Human. This Primary Antibody has been cited in 3+ publications.

Quick Overview for XBP1 antibody (ABIN969462)

Target: XBP1 (X-Box Binding Protein 1 (XBP1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This XBP1 antibody is un-conjugated

Application: ELISA

Clone: 1C4

Product Details anti-XBP1 Antibody

Purpose: XBP1 Antibody

Purification: Ascitic fluid

Immunogen: Purified recombinant fragment of human XBP1 expressed in E. Coli.

Isotype: IgG1

Application Details

Application Notes: ELISA: 1/10000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Ascitic fluid containing 0.03 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

References for anti-XBP1 antibody (ABIN969462)

Martino, Olsen, Fulcher, Wolfgang, ONeal, Ribeiro: "Airway epithelial inflammation-induced endoplasmic reticulum Ca2+ store expansion is mediated by X-box binding protein-1." in: The Journal of biological chemistry, Vol. 284, Issue 22, pp. 14904-13, (2009) (PubMed).

Schardt, Weber, Eyholzer, Mueller, Pabst: "Activation of the unfolded protein response is associated with favorable prognosis in acute myeloid leukemia." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 15, Issue 11, pp. 3834-41, (2009) (PubMed).

Jiang, Yang, Thorne, Zhu, Hersey, Zhang: "Human melanoma cells under endoplasmic reticulum stress acquire resistance to microtubule-targeting drugs through XBP-1-mediated activation of Akt." in: Neoplasia (New York, N.Y.), Vol. 11, Issue 5, pp. 436-47, (2009) (PubMed).

Target Details for XBP1

Target: XBP1 (X-Box Binding Protein 1 (XBP1))

Alternative Name: XBP1

Background: This gene encodes a transcription factor that regulates MHC class II genes by binding to a promoter element referred to as an X box. This gene product is a bZIP protein, which was also identified as a cellular transcription factor that binds to an enhancer in the promoter of the T cell leukemia virus type 1 promoter. It may increase expression of viral proteins by acting as the DNA binding partner of a viral transactivator. It has been found that upon accumulation of unfolded proteins in the endoplasmic reticulum (ER), the mRNA of this gene is processed to an active form by an unconventional splicing mechanism that is mediated by the endonuclease inositol-requiring enzyme 1 (IRE1). The resulting loss of 26 nt from the spliced mRNA causes a frame-shift and an isoform XBP1(S), which is the functionally active transcription factor. The isoform encoded by the unspliced mRNA, XBP1(U), is constitutively expressed, and thought to function as a negative feedback regulator of XBP1(S), which shuts off transcription of target genes during the recovery phase of ER stress. A pseudogene of XBP1 has been identified and localized to chromosome 5.

Molecular Weight: 28 kDa

Gene ID: 7494

UniProt: P17861

Pathways: ER-Nucleus Signaling, Unfolded Protein Response