EIF4B antibody

Catalog No. ABIN969517

This Mouse Monoclonal antibody specifically detects EIF4B in WB, ELISA, IHC, FACS and ICC. It exhibits reactivity toward Human and Mouse and has been mentioned in 2+ publications.

Quick Overview for EIF4B antibody (ABIN969517)

Target: EIF4B (Eukaryotic Translation Initiation Factor 4B (EIF4B))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This EIF4B antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Clone: 1F5

Product Details anti-EIF4B Antibody

Purpose: EIF4B Antibody

Purification: Purified antibody

Immunogen: Purified recombinant fragment of human EIF4B expressed in E. Coli.

Isotype: IgG2b

Application Details

Application Notes:

ELISA: 1/10000

FCM: 1/200 - 1/400

ICC: 1/200 - 1/1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified antibody in PBS with 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

References for anti-EIF4B antibody (ABIN969517)

Mokas, Mills, Garreau, Fournier, Robert, Arya, Kaufman, Pelletier, Mazroui: "Uncoupling stress granule assembly and translation initiation inhibition." in: Molecular biology of the cell, Vol. 20, Issue 11, pp. 2673-83, (2009) (PubMed).

Shahbazian, Roux, Mieulet, Cohen, Raught, Taunton, Hershey, Blenis, Pende, Sonenberg: "The mTOR/PI3K and MAPK pathways converge on eIF4B to control its phosphorylation and activity." in: The EMBO journal, Vol. 25, Issue 12, pp. 2781-91, (2006) (PubMed).

Target Details for EIF4B

Target: EIF4B (Eukaryotic Translation Initiation Factor 4B (EIF4B))

Alternative Name: EIF4B

Background: The eukaryotic translation initiation factor 4B (eIF4B) plays a critical role in recruiting the 40S ribosomal subunit to the mRNA.It functions in close association with eIF4F and eIF4A. It binds near the 5'-terminal cap of mRNA in the presence of eIF4F and ATP. It promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both eIF4A and eIF4F

Molecular Weight: 80 kDa

Gene ID: 1975

UniProt: P23588