MELK antibody (AA 637-651)

Catalog No. ABIN969538

This Mouse Monoclonal antibody specifically detects MELK in ELISA, IHC, ICC and FACS. It exhibits reactivity toward Human and has been mentioned in 2+ publications.

Quick Overview for MELK antibody (AA 637-651) (ABIN969538)

Target: MELK (Maternal Embryonic Leucine Zipper Kinase (MELK))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MELK antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Clone: 2G2

Product Details anti-MELK Antibody

Binding Specificity: AA 637-651

Purpose: MELK Antibody

Sequence: VYKRLVEDIL SSCKV

Purification: Purified antibody

Immunogen: Synthesized peptide of human MELK(AA: 637-651:C-VYKRLVEDILSSCKV).

Isotype: IgG2a

Application Details

Application Notes:

ELISA: 1/10000

FCM: 1/200 - 1/400

ICC: 1/200 - 1/1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified antibody in PBS with 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

References for anti-MELK antibody (ABIN969538)

Nakano, Joshi, Visnyei, Hu, Watanabe, Lam, Wexler, Saigusa, Nakamura, Laks, Mischel, Viapiano, Kornblum: "Siomycin A targets brain tumor stem cells partially through a MELK-mediated pathway." in: Neuro-oncology, Vol. 13, Issue 6, pp. 622-34, (2011) (PubMed).

Pickard, Green, Ellis, Caldas, Hedge, Mourtada-Maarabouni, Williams: "Dysregulated expression of Fau and MELK is associated with poor prognosis in breast cancer." in: Breast cancer research : BCR, Vol. 11, Issue 4, pp. R60, (2009) (PubMed).

Target Details for MELK

Target: MELK (Maternal Embryonic Leucine Zipper Kinase (MELK))

Alternative Name: MELK

Background: Maternal embryonic leucine-zipper kinase (MELK) is a key regulator of survival of stemlike GBM cells in vitro. MELK expression is increased in breast cancer tissue and this increase is also associated with poor patient survival, as predicted for a candidate oncogene.

Molecular Weight: 74.6 kDa

Gene ID: 9833

UniProt: Q14680