NCK1 antibody

Catalog No. ABIN969545

The Mouse Monoclonal anti-NCK1 antibody has been validated for WB, IHC, ELISA and FACS. It is suitable to detect NCK1 in samples from Human and Monkey. There are 2+ publications available.

Quick Overview for NCK1 antibody (ABIN969545)

Target: NCK1 (NCK Adaptor Protein 1 (NCK1))

Reactivity: Human, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This NCK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

Clone: 5B7

Product Details anti-NCK1 Antibody

Purpose: NCK1 Antibody

Purification: Purified antibody

Immunogen: Purified recombinant fragment of human NCK1 expressed in E. Coli.

Isotype: IgG1

Application Details

Application Notes:

ELISA: 1/10000

FCM: 1/200 - 1/400

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified antibody in PBS with 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

References for anti-NCK1 antibody (ABIN969545)

Preisinger, Kolch: "The Bcr-Abl kinase regulates the actin cytoskeleton via a GADS/Slp-76/Nck1 adaptor protein pathway." in: Cellular signalling, Vol. 22, Issue 5, pp. 848-56, (2010) (PubMed).

Blasutig, New, Thanabalasuriar, Dayarathna, Goudreault, Quaggin, Li, Gruenheid, Jones, Pawson: "Phosphorylated YDXV motifs and Nck SH2/SH3 adaptors act cooperatively to induce actin reorganization." in: Molecular and cellular biology, Vol. 28, Issue 6, pp. 2035-46, (2008) (PubMed).

Target Details for NCK1

Target: NCK1 (NCK Adaptor Protein 1 (NCK1))

Alternative Name: NCK1

Background: The protein encoded by this gene is one of the signaling and transforming proteins containing Src homology 2 and 3 (SH2 and SH3) domains. It is located in the cytoplasm and is an adaptor protein involved in transducing signals from receptor tyrosine kinases to downstream signal recipients such as RAS. Alternatively spliced transcript variants encoding different isoforms have been found.

Molecular Weight: 47 kDa

UniProt: P16333

Pathways: TCR Signaling, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals