NEFL antibody

Catalog No. ABIN969548

The Mouse Monoclonal anti-NEFL antibody has been validated for WB, IHC, ELISA, FACS and ICC. It is suitable to detect NEFL in samples from Human. There are 2+ publications available.

Quick Overview for NEFL antibody (ABIN969548)

Target: NEFL (Neurofilament, Light Polypeptide (NEFL))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This NEFL antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC)

Clone: 1H3

Product Details anti-NEFL Antibody

Purpose: NEFL Antibody

Purification: Ascitic fluid

Immunogen: Purified recombinant fragment of human NEFL expressed in E. Coli.

Isotype: IgG1

Application Details

Application Notes:

ELISA: 1/10000

FCM: 1/200 - 1/400

ICC: 1/200 - 1/1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Ascitic fluid containing 0.03 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

References for anti-NEFL antibody (ABIN969548)

Lee, Kim, Chung, Kim, Kwon, Min, Chang: "The effect of rod domain A148V mutation of neurofilament light chain on filament formation." in: BMB reports, Vol. 41, Issue 12, pp. 868-74, (2009) (PubMed).

Abe, Numakura, Saito, Koide, Oka, Honma, Kishikawa, Hayasaka: "Neurofilament light chain polypeptide gene mutations in Charcot-Marie-Tooth disease: nonsense mutation probably causes a recessive phenotype." in: Journal of human genetics, Vol. 54, Issue 2, pp. 94-7, (2009) (PubMed).

Target Details for NEFL

Target: NEFL (Neurofilament, Light Polypeptide (NEFL))

Alternative Name: NEFL

Background: Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. Mutations in this gene cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y.

Molecular Weight: 62 kDa

Gene ID: 4747

UniProt: P07196