PPM1A antibody (AA 202-382)

Catalog No. ABIN969556

This Mouse Monoclonal antibody specifically detects PPM1A in WB, ELISA and FACS. It exhibits reactivity toward Human and Monkey and has been mentioned in 2+ publications.

Quick Overview for PPM1A antibody (AA 202-382) (ABIN969556)

Target: PPM1A (Protein Phosphatase, Mg2+/Mn2+ Dependent, 1A (PPM1A))

Reactivity: Human, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This PPM1A antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Clone: 7F12H6

Product Details anti-PPM1A Antibody

Binding Specificity: AA 202-382

Purpose: PPM1A Antibody

Purification: Purified antibody

Immunogen: Purified recombinant fragment of human PPM1A (AA: 202-382) expressed in E. Coli.

Isotype: IgG1

Application Details

Application Notes:

ELISA: 1/10000

FCM: 1/200 - 1/400

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified antibody in PBS with 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

References for anti-PPM1A antibody (ABIN969556)

Malki, Uher, Paya-Cano, Binder, Rietschel, Zobel, Mors, Hauser, Henigsberg, Jerman, Souery, Placentino, Ng, Cohen-Woods, Sluyter, Farmer, Aitchison, Craig, Lewis, McGuffin, Schalkwyk: "Convergent animal and human evidence suggests a role of PPM1A gene in response to antidepressants." in: Biological psychiatry, Vol. 69, Issue 4, pp. 360-5, (2011) (PubMed).

Sun, Yu, Dotti, Shen, Tan, Savoldo, Pass, Chu, Zhang, Lu, Fu, Lin, Yang: "PPM1A and PPM1B act as IKKbeta phosphatases to terminate TNFalpha-induced IKKbeta-NF-kappaB activation." in: Cellular signalling, Vol. 21, Issue 1, pp. 95-102, (2008) (PubMed).

Target Details for PPM1A

Target: PPM1A (Protein Phosphatase, Mg2+/Mn2+ Dependent, 1A (PPM1A))

Alternative Name: PPM1A

Background: The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase dephosphorylates, and negatively regulates the activities of, MAP kinases and MAP kinase kinases. It has been shown to inhibit the activation of p38 and JNK kinase cascades induced by environmental stresses. This phosphatase can also dephosphorylate cyclin-dependent kinases, and thus may be involved in cell cycle control. Overexpression of this phosphatase is reported to activate the expression of the tumor suppressor gene TP53/p53, which leads to G2/M cell cycle arrest and apoptosis. Three alternatively spliced transcript variants encoding distinct isoforms have been described.

Molecular Weight: 42.4 kDa

Gene ID: 5494

UniProt: P20265