Elastase antibody

Catalog No. ABIN98664

Supplier Product No.: 100-4168

This anti-Elastase antibody is a Rabbit Polyclonal antibody detecting Elastase in ELISA, WB and IHC. Suitable for Pig.

Quick Overview for Elastase antibody (ABIN98664)

Target: Elastase

Reactivity: Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Elastase antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Elastase Antibody

Supplier Product No.: 100-4168

Supplier: Rockland

Purpose: Elastase Antibody

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, purified and partially purified Elastase [Porcine Pancreas].

Characteristics: Synonyms: rabbit anti-Elastase Antibody, Fecal pancreatic elastase 1 antibody, HLE antibody, Medullasin antibody, Pancreatic elastase IIA antibody, PE 1 antibody, PE1 antibody, Protease E antibody

Purification: Elastase antibody was prepared from monospecific antiserum by a delipidation and defibrination.

Immunogen:

Immunogen: Elastase [Porcine Pancreas]

Immunogen Type: Native Protein

Application Details

Application Notes:

Immunohistochemistry Dilution: User Optimized

Application Note: Elastase antibody is suitable for ELISA and western blot. Usage in other immunoassays such as immunohistochemistry is expected but has not been determined. Optimal concentrations should be determined by researcher.

Western Blot Dilution: 1:2,000 - 1:10,000

ELISA Dilution: 1:20,000 - 1:100,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 2.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 80 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for Elastase

Target: Elastase

Background: Background: Anti elastase antibody detects elastase enzyme. Elastase breaks down elastin, an elastic fiber that, together with collagen, determines the mechanical properties of connective tissue. The specific peptide bonds cleaved are those on the carboxy side of small, hydrophobic amino acids such as glycine, alanine, and valine.

Gene ID: 396766

NCBI Accession: NP_998988

UniProt: P00772