ZAP70 antibody (Internal Region)

Catalog No. ABIN987274

This Goat Polyclonal antibody specifically detects ZAP70 in WB, ELISA and IP. It exhibits reactivity toward Human.

Quick Overview for ZAP70 antibody (Internal Region) (ABIN987274)

Target: ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This ZAP70 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Grade: Verified

Product Details anti-ZAP70 Antibody

Binding Specificity: Internal Region

Purpose: ZAP70

Sequence: SDGYTPEPAR ITSPD

Specificity: This antibody is expected to recognize isoform 1 (NP_001070.2) only.

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-SDGYTPEPARITSPD, from the internal region of the protein sequence according to NP_001070.2.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 70 kDa band observed in lysates of cell lines Jurkat and Molt4 (calculated MW of 69.9 kDa according to NP_001070.2). Recommended concentration: 0.3-1 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Immunoprecipitation:This antibody was deemed fit for IP under native conditions (observations from customer).

Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (201

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for ZAP70

Target: ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

Alternative Name: ZAP70

Background: ZAP70, zeta-chain (TCR) associated protein kinase 70 kDa, FLJ17670, FLJ17679, SRK, STD, TZK, ZAP-70, 70 kDa zeta-associated protein, syk-related tyrosine kinase, tyrosine-protein kinase ZAP-70, zeta-chain associated protein kinase, 70kD

Gene ID: 7535, 22637, 301348

NCBI Accession: NP_001070

Pathways: TCR Signaling, Ubiquitin Proteasome Pathway