CES3 antibody

Catalog No. ABIN98993

Supplier Product No.: 200-4169-0100

The Rabbit Polyclonal anti-CES3 antibody has been validated for WB and ELISA. It is suitable to detect CES3 in samples from Pig.

Quick Overview for CES3 antibody (ABIN98993)

Target: CES3 (Carboxylesterase 3 (CES3))

Reactivity: Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CES3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-CES3 Antibody

Supplier Product No.: 200-4169-0100

Supplier: Rockland

Purpose: Esterase Antibody

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Esterase [Porcine Liver].

Characteristics: Synonyms: rabbit anti-Esterase Antibody, Liver carboxylesterase, Proline-beta-naphthylamidase, Retinyl ester hydrolase

Purification: Anti-Esterase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Esterase [Porcine Liver]

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti-Esterase is suitable for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: 1:1,000 - 1:5,000

ELISA Dilution: 1:10,000 - 1:50,000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for CES3

Target: CES3 (Carboxylesterase 3 (CES3))

Alternative Name: CES3

Background: Background: Esterase is involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. It is active towards triacylglycerides containing short-chain fatty acids from C2 to C6, and 13-monoacylglycerols containing fatty acids from C2 to C12, and inactive on long-chain triacylglycerols and diacylglycerol. it hydrolyzes aromatic and alkyl esters and vitamin A acetate. The hydrolysis rate depends upon the amino acid promoiety and the esterification site of the prodrug. Aromatic promoieties are favored, highest rates are observed with phenylalanyl progdrugs, hydrolysis of valyl and isoleucyl prodrugs is less efficient. With floxuridine prodrugs, activity is higher on 5' monoesters than on 3' monoesters, while with gemcitabine prodrugs, activity is higher on 3' monoesters than on 5' monoesters.

Gene ID: 100626873, 343791015

UniProt: Q29550

Pathways: Monocarboxylic Acid Catabolic Process