Fluorescein antibody

Catalog No. ABIN99340

Supplier Product No.: 600-101-096

This Goat Polyclonal antibody specifically detects in WB, ELISA, IHC and FACS. It exhibits reactivity toward and has been mentioned in 9+ publications.

Quick Overview for Fluorescein antibody (ABIN99340)

Target: Fluorescein

Host: Goat

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details

Supplier Product No.: 600-101-096

Supplier: Rockland

Purpose: Fluorescein (FITC) Antibody

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and Fluorescein conjugated Bovine Serum Albumin.

Characteristics: Synonyms: goat Anti FITC, goat anti Fluorescein

Purification: Fluorescein Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Fluorescein conjugated Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Sterility: Sterile filtered

Immunogen:

Immunogen: Fluorescein conjugated to Goat IgG

Immunogen Type: Other

Isotype: IgG

Application Details

Application Notes:

Flow Cytometry Dilution: User Optimized

Immunohistochemistry Dilution: 1:250 - 1:1,500

Application Note: Anti-Fluorescein Antibody has been tested by ELISA and western blot and is suitable for immunomicroscopy, in situ hybridization and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.

Western Blot Dilution: 1:500 - 1:3,000

ELISA Dilution: 1:5,000 - 1:25,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to opening.  Aliquot contents and freeze at -20° C or below for extended storage.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

References

Jacobsen, Yi, Al Asafen, Jermusyk, Beisel, Reeves: "Tunable self-cleaving ribozymes for modulating gene expression in eukaryotic systems." in: PLoS ONE, Vol. 15, Issue 4, pp. e0232046, (2020) (PubMed).

Al Asafen, Bandodkar, Carrell-Noel, Schloop, Friedman, Reeves: "Robustness of the Dorsal morphogen gradient with respect to morphogen dosage." in: PLoS computational biology, Vol. 16, Issue 4, pp. e1007750, (2020) (PubMed).

Kotani, Yamaguchi, Ohnishi, Kuwahara, Nakano, Nakano, Ida, Murakoshi, Honke: "Proximity proteomics identifies cancer cell membrane cis-molecular complex as a potential cancer target." in: Cancer science, Vol. 110, Issue 8, pp. 2607-2619, (2019) (PubMed).

Liu, Zheng: "Bach1 siRNA attenuates bleomycin-induced pulmonary fibrosis by modulating oxidative stress in mice." in: International journal of molecular medicine, Vol. 39, Issue 1, pp. 91-100, (2017) (PubMed).

Fu, Zheng, Dong, Wang, Jiang: "Activation of cannabinoid receptor type 2 by JWH133 alleviates bleomycin-induced pulmonary fibrosis in mice." in: Oncotarget, Vol. 8, Issue 61, pp. 103486-103498, (2017) (PubMed).

Guest, Ilic, Scrable, Sell: "Survival of irradiated recipient mice after transplantation of bone marrow from young, old and "early aging" mice." in: Aging, Vol. 7, Issue 12, pp. 1212-23, (2016) (PubMed).

Miyagawa-Yamaguchi, Kotani, Honke: "Expressed glycosylphosphatidylinositol-anchored horseradish peroxidase identifies co-clustering molecules in individual lipid raft domains." in: PLoS ONE, Vol. 9, Issue 3, pp. e93054, (2015) (PubMed).

Guest, Ilic, Ma, Grant, Glinsky, Sell: "Direct and indirect contribution of bone marrow-derived cells to cancer." in: International journal of cancer, Vol. 126, Issue 10, pp. 2308-18, (2010) (PubMed).

Noga, Udomkusonsri: "Fluorescein: a rapid, sensitive, nonlethal method for detecting skin ulceration in fish." in: Veterinary pathology, Vol. 39, Issue 6, pp. 726-31, (2002) (PubMed).

Target Details

Target: Fluorescein

Target Type: Chemical

Background: Background: Anti-Fluorescein Antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. Anti-FITC is also suitable as a visualization reagent for fluorescein-labelled compounds (such as antibodies, lectins and peptide nucleic acid (PNA) probes).