Fluorescein antibody (Texas Red (TR))

Catalog No. ABIN99347

Supplier Product No.: 600-109-096

Product Details

Target: Fluorescein

Host: Goat

Clonality: Polyclonal

Conjugate: Texas Red (TR)

Application: Western Blotting (WB), Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)

Supplier Product No.: 600-109-096

Supplier: Rockland

Purpose: Fluorescein Antibody Texas Red™ Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and Fluorescein conjugated Bovine Serum Albumin.

Characteristics: Synonyms: goat Anti-FITC antibody Texas Red™ conjugation, Texas Red™ conjugated goat anti-fluorescein antibody, Texas Red™ conjugated goat Anti-FITC antibody, goat anti-fluorescein antibody Texas Red™ conjugation

Purification: Fluorescein Antibody Texas Red™ Conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using Fluorescein IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Immunogen:

Immunogen: Fluorescein conjugated to Goat IgG

Immunogen Type: Other

Isotype: IgG

Labeling Ratio: 2.9

Application Details

Application Notes:

Flow Cytometry Dilution: User Optimized

Application Note: Anti-Fluorescein Antibody Texas Red™ Conjugated is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.

Western Blot Dilution: >1:10,000

FLISA Dilution: >1:20,000

IF Microscopy Dilution: 1:500 - 1:2,500

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

Target Details

Target: Fluorescein

Target Type: Chemical

Background: Background: Fluorescein Antibody Texas Red™ Conjugated is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.