Fluorescein antibody (Rhodamine)
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- Target
- Fluorescein
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- Rhodamine
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Application
- Western Blotting (WB), FLISA, Fluorescence Microscopy (FM)
- Supplier Product No.
- 600-100-096
- Supplier
- Rockland
- Purpose
- Fluorescein Antibody Rhodamine Conjugated
- Cross-Reactivity (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and Fluorescein Conjugated IgG.
- Characteristics
- Synonyms: goat Anti-FITC antibody Rhodamine conjugation, TRITC conjugated goat anti-fluorescein antibody, TRITC conjugated goat Anti-FITC antibody, goat anti-fluorescein antibody Rhodamine conjugation
- Purification
- Fluorescein Antibody Conjugated to Rhodamine was prepared from monospecific antiserum by immunoaffinity chromatography using Fluorescein IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
- Immunogen
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Immunogen: Fluorescein conjugated to Goat IgG
Immunogen Type: Other
- Isotype
- IgG
- Labeling Ratio
- 2.0
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- Application Notes
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Application Note: Anti-Fluorescein Rhodamine Conjugated Antibody is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Western Blot Dilution: >1:10,000
FLISA Dilution: >1:20,000
IF Microscopy Dilution: 1:500 - 1:2,500
Other: User Optimized
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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Reconstitution Volume: 1.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- Concentration
- 1.0 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide - Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Expiry Date
- 12 months
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- Target
- Fluorescein
- Target Type
- Chemical
- Background
- Background: Anti-Fluorescein Rhodamine Conjugated Antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
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