Fluorescein antibody (Rhodamine)

Catalog No. ABIN99348

Supplier Product No.: 600-100-096

Product Details

Target: Fluorescein

Host: Goat

Clonality: Polyclonal

Conjugate: Rhodamine

Application: Western Blotting (WB), FLISA, Fluorescence Microscopy (FM)

Supplier Product No.: 600-100-096

Supplier: Rockland

Purpose: Fluorescein Antibody Rhodamine Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and Fluorescein Conjugated IgG.

Characteristics: Synonyms: goat Anti-FITC antibody Rhodamine conjugation, TRITC conjugated goat anti-fluorescein antibody, TRITC conjugated goat Anti-FITC antibody, goat anti-fluorescein antibody Rhodamine conjugation

Purification: Fluorescein Antibody Conjugated to Rhodamine was prepared from monospecific antiserum by immunoaffinity chromatography using Fluorescein IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Immunogen:

Immunogen: Fluorescein conjugated to Goat IgG

Immunogen Type: Other

Isotype: IgG

Labeling Ratio: 2.0

Application Details

Application Notes:

Application Note: Anti-Fluorescein Rhodamine Conjugated Antibody is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.

Western Blot Dilution: >1:10,000

FLISA Dilution: >1:20,000

IF Microscopy Dilution: 1:500 - 1:2,500

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

Target Details

Target: Fluorescein

Target Type: Chemical

Background: Background: Anti-Fluorescein Rhodamine Conjugated Antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.