Fructosyl Amino Acid Oxidase antibody

Catalog No. ABIN99448

Supplier Product No.: 200-601-236-0100

The Sheep Polyclonal anti-Fructosyl Amino Acid Oxidase antibody has been validated for WB, IP and ELISA. It is suitable to detect Fructosyl Amino Acid Oxidase in samples from Corynebacterium.

Quick Overview for Fructosyl Amino Acid Oxidase antibody (ABIN99448)

Target: Fructosyl Amino Acid Oxidase (socD)

Reactivity: Corynebacterium

Host: Sheep

Clonality: Polyclonal

Conjugate: This Fructosyl Amino Acid Oxidase antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), ELISA

Product Details anti-Fructosyl Amino Acid Oxidase Antibody

Supplier Product No.: 200-601-236-0100

Supplier: Rockland

Purpose: Fructosyl-Amino Acid Oxidase Antibody

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Sheep Serum as well as purified and partially purified Fructosyl-Amino Acid Oxidase [E.

Characteristics: Synonyms: sheep anti-Fructosyl-Amino Acid Oxidase Antibody, Fructosyl-amino acid oxidase

Purification: Anti-FRUCTOSYL-AMINO ACID OXIDASE is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Fructosyl-Amino Acid Oxidase [from Corynebacterium sp. Expressed in E.coli]

Immunogen Type: Recombinant Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: Fructosyl-amino acid oxidase antibody has been tested by western blot and is suitable to be assayed against 1.0 μg of Fructosyl-Amino Acid Oxidase [E.coli] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Sheep IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:2,000 to 1:8,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500 - 1:5,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:5,000 - 1:20,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

Target Details for Fructosyl Amino Acid Oxidase

Target: Fructosyl Amino Acid Oxidase (socD)

Alternative Name: Fructosyl-Amino Acid Oxidase

Background: Background: Fructosyl amino acid oxidase is a flavoprotein that catalyzes the oxidation of fructosyl amino acids to form glucosone, amino acid and hydrogen peroxide. Anti-Fructosyl-amino acid oxidase can be useful for researchers interested in diabetes research.

UniProt: Q8RIU8