Glucose-6-Phosphate Dehydrogenase antibody

Catalog No. ABIN99693

Supplier Product No.: 100-101-214

Product Details anti-Glucose-6-Phosphate Dehydrogenase Antibody

Target: Glucose-6-Phosphate Dehydrogenase (G6PD)

Reactivity: Bacillus

Host: Goat

Clonality: Polyclonal

Conjugate: This Glucose-6-Phosphate Dehydrogenase antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Supplier Product No.: 100-101-214

Supplier: Rockland

Purpose: Glucose Dehydrogenase Antibody

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, purified and partially purified Glucose Dehydrogenase [Bacillus].

Characteristics: Synonyms: goat anti-Glucose Dehydrogenase Antibody, gdh antibody, gdh2 antibody, gdh3 antibody, gdh4 antibody, gdhI antibody, GLCDHIII antibody, GLCDHIV antibody, Glucose 1 dehydrogenase 1 antibody

Purification: This product was prepared from monospecific antiserum by a delipidation and defibrination.

Immunogen:

Immunogen: Glucose Dehydrogenase [Bacillus]

Immunogen Type: Native Protein

Application Details

Application Notes:

Application Note: Anti-Glucose Dehydrogenase has been tested by western blot and is suitable to be assayed against 1.0 μg of Glucose Dehydrogenase [Bacillus] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:7,500 to 1:32,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500 - 1:3,000

ELISA Dilution: 1:5,000 - 1:25,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 2.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 90 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for Glucose-6-Phosphate Dehydrogenase

Target: Glucose-6-Phosphate Dehydrogenase (G6PD)

Alternative Name: Glucose Dehydrogenase (G6PD Products)

Background: Background: Glucose Dehydrogenase catalytic activity: D-glucose + NAD(P)+ = D-glucono-1,5-lactone + NAD(P)H.

Gene ID: 8985276

UniProt: P07999

Pathways: Regulation of Systemic Arterial Blood Pressure by Hormones