Glycerol Kinase antibody

Catalog No. ABIN99889

Supplier Product No.: 100-101-211

Product Details anti-Glycerol Kinase Antibody

Target: Glycerol Kinase (GK)

Reactivity: Cellulomonas

Host: Goat

Clonality: Polyclonal

Conjugate: This Glycerol Kinase antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Supplier Product No.: 100-101-211

Supplier: Rockland

Purpose: Glycerol Kinase Antibody

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, purified and partially purified Glycerol Kinase [Cellulomonas species].

Characteristics: Synonyms: goat anti-Glycerol Kinase Antibody, ATP glycerol 3 phosphotransferase antibody, GK antibody, GK1 antibody, GKD antibody, Glycerokinase antibody, Glycerol kinase antibody, Glycerol kinase deficiency antibody

Purification: This product was prepared from monospecific antiserum by a delipidation and defibrination.

Immunogen:

Immunogen: Glycerol Kinase [Cellulomonas species]

Immunogen Type: Native Protein

Application Details

Application Notes:

Application Note: Anti-Glycerol Kinase has been tested by western blot and is suitable to be assayed against 1.0 μg of Glycerol Kinase [Celullomonas species] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) code #605-4302 and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:1,000 to 1:2,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500 - 1:2,000

ELISA Dilution: 1:4,000 - 1:20,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 2.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 90 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

Target Details for Glycerol Kinase

Target: Glycerol Kinase (GK)

Alternative Name: glpK (GK Products)

Background: Background: Glycerol Kinase is the key enzyme in the regulation of glycerol uptake and metabolism. It catalyzes the phosphorylation of glycerol to yield sn-glycerol 3-phosphate. It is inhibited by fructose 1,6-bisphosphate (FBP). This protein is involved in step 1 of the subpathway that synthesizes sn-glycerol 3-phosphate from glycerol.

Gene ID: 6960048

UniProt: D0VZG4