Western Blotting (WB), Immunohistochemistry (IHC), ELISA
Cross-Reactivity (Details)
Cross reactivity against a-1-Acid Glycoprotein from other tissues and species may occur but has not been specifically determined.
Purification
Alpha-1-Acid Glycoprotein Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified a-1-Acid Glycoprotein (Human Plasma).
Endotoxin Level
Low Endotoxin : No
Immunogen
Anti-Alpha-1-Acid Glycoprotein Antibody was produced by repeated immunizations with human plasma a-1-Acid Glycoprotein. Immunogen Type: Native Protein
Anti-Alpha-1-Acid Glycoprotein Antibody is suitable for western blottingand for ELISA. Researchers should determine optimal titers for applications that are not stated below. ELISA Dilution: 1:135.000 Western Blot Dilution: 1:2.000 - 1:10.000
Restrictions
For Research Use only
Format
Liquid
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Handling Advice
Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Storage
-20 °C
Storage Comment
Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.
Expiry Date
Expiration date is one (1) year from date of opening.
Anti-Alpha-1-Acid Glycoprotein Antibody detects Alpha-1-Acid Glycoprotein. A-1-Acid Glycoprotein is expressed by the liver and secreted in plasma. It functions as a transport protein in the blood stream. It binds various ligands in the interior of its beta-barrel domain. It binds synthetic drugs and influences their distribution and availability in the body. It appears to function in modulating the activity of the immune system during the acute-phase reaction. Anti-Alpha-1-Acid Glycoprotein Antibody is ideal for investigators involved in Cell Signaling, Neuroscience and Signal Transduction research. Synonyms: agpa antibody, Alpha 1 acid glycoprotein antibody, Alpha 1 agp antibody, Glycoprotein alpha acid of serum antibody, OMD 1 antibody, omd1 antibody, orm antibody, ORM1 antibody, Orosomucoid 1 antibody