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Western Blot Antibodies

Western blot (WB) antibodies are optimized for the detection of denatured proteins and validated to deliver clear, specific bands at the expected molecular weight. Use the filters below to quickly identify primary antibodies matched to your target, species, and experimental requirements.

Western Blot Antibody Validation

Antibodies for Western blot must reliably detect target proteins under denaturing conditions. Products in this category are validated for WB applications and selected for specific band detection at the expected molecular weight. Where available, validation data include experimental images, independent testing, and literature references to support antibody performance.

Western Blot Considerations

Protein denaturation during Western blotting can alter epitope structure, making antibody selection critical. Antibodies validated for WB are optimized to recognize linearized epitopes and reduce non-specific binding.

Monoclonal antibodies provide high specificity and consistent results, while polyclonal antibodies can offer stronger signal due to multi-epitope recognition. Recombinant antibodies combine defined specificity with improved reproducibility across experiments.

Western Blotting: Principles, Protocols, and Troubleshooting

Learn more about Western blotting, including protocols and troubleshooting

Use the filters below to narrow down Western blot antibodies by target, species, and experimental requirements.

515,351 results

CD163 Reactivity: Human WB, FACS, ELISA, ICC, IHC (p), IHC (fro), IF (cc), IF (p) Host: Rabbit Polyclonal unconjugated
Pubmed 27 references
Catalog No. ABIN741570
$384.62
Plus shipping costs $50.00
100 μL
Shipping to: United States
Delivery in 4 to 6 Business Days
 
CBS Reactivity: Human WB, ELISA, IHC (p), IP, RNAi Host: Mouse Monoclonal 3E1 unconjugated
Pubmed 38 references
Catalog No. ABIN7489109
$489.14
Plus shipping costs $50.00
50 μg
Shipping to: United States
Delivery in 11 to 12 Business Days
 
DYRK1A Reactivity: Human WB, ELISA Host: Mouse Monoclonal 7D10 unconjugated
Pubmed 39 references
Catalog No. ABIN560675
$489.14
Plus shipping costs $50.00
100 μg
Shipping to: United States
Delivery in 11 to 12 Business Days
 
MAP1LC3A Reactivity: Human, Mouse WB, IF, ICC, IHC (p) Host: Rabbit Polyclonal RB38908 unconjugated
Pubmed 35 references
Catalog No. ABIN388483
$451.00
Plus shipping costs $50.00
400 μL (More quantities available)
Shipping to: United States
Delivery in 10 to 11 Business Days
 
NLRP3 Reactivity: Human, Mouse WB, IHC, IF, IP Host: Mouse Monoclonal Cryo-2 unconjugated
Pubmed 60 references
Catalog No. ABIN1169100
$590.53
Plus shipping costs $50.00
100 μg
Shipping to: United States
Delivery in 5 to 8 Business Days
 
AGO2 Reactivity: Human WB, ELISA, IF, IHC (p), RNAi Host: Mouse Monoclonal 2E12-1C9 unconjugated
Pubmed 44 references
Catalog No. ABIN565346
$520.15
Plus shipping costs $50.00
100 μg
Shipping to: United States
Delivery in 11 to 12 Business Days
 
HSP70 Reactivity: Human WB, IHC, ELISA, IF, ICC, FACS, IEM, AA, BI Host: Mouse Monoclonal C92F3A-5 unconjugated
Pubmed 38 references
Catalog No. ABIN361708
$581.25
Plus shipping costs $50.00
200 μg
Shipping to: United States
Delivery in 3 to 4 Business Days
 
ACTB Reactivity: Mouse, Rat, Chicken, Zebrafish (Danio rerio) WB, ICC, IP Host: Rabbit Polyclonal unconjugated
Pubmed 4 references Independent Validation 1 validation
Catalog No. ABIN1742508
$550.56
Plus shipping costs $50.00
100 μg
Shipping to: United States
Delivery in 5 to 6 Business Days
Independently Validated
Rockland 600-401-253 APP Reactivity: Human, Mouse WB, ELISA, IHC, IF, FM Host: Rabbit Polyclonal unconjugated
Pubmed 1 reference Independent Validation 2 validations
Catalog No. ABIN95037
$484.00
Plus shipping costs $50.00, if applicable $20.00 dry ice
100 μg (More quantities available)
Shipping to: United States
Delivery in 1 to 2 Business Days
Independently Validated
Rockland 600-401-105-0.1 COL3A1 Reactivity: Human, Cow, Pig WB, IHC, ELISA, IP, FACS, DB, FM Host: Rabbit Polyclonal unconjugated
Pubmed 5 references Independent Validation 1 validation
Catalog No. ABIN5596830
$522.00
Plus shipping costs $50.00
100 μg
Shipping to: United States
Delivery in 1 to 2 Business Days
Independently Validated
  • Type Primary
    • Primary
  • Target
    • Histone 3 (H3)
    • p53 (TP53)
    • EGFR
    • tau
    • AKT1
    • NF-kB p65 (NFkBP65)
    • Estrogen Receptor alpha (ESR1)
    • Bcl-2 (BCL2)
    • KIT
    • TNF alpha
    • RAF1
    • c-MYC (MYC)
    • FAK (PTK2)
    • GFAP
    • Vimentin (VIM)
    • RPS6KB1
    • HSP27 (HSPB1)
    • ErbB2/Her2
    • HSP70
    • Albumin (ALB)
    • Cytokeratin 18 (KRT18)
    • C-JUN (JUN)
    • IL-6 (IL6)
    • CDK1
    • Histone H4
    • ATF2
    • STAT3
    • EpCAM (EPCAM)
    • SMAD2
    • SNCA
    • Caspase 9 (CASP9)
    • Cytokeratin 19 (KRT19)
    • MEK1 (MAP2K1)
    • Interferon gamma (IFNG)
    • GAPDH
    • beta Catenin (CATNB)
    • HSPD1
    • CREB1
    • SOD1
    • BAD
    • Src
    • Caspase 3 (CASP3)
    • CD31 (PECAM1)
    • ERK1 (MAPK3)
    • E-cadherin (CDH1)
    • GRP78 (HSPA5)
    • eIF4EBP1 (EIF4EBP1)
    • FGFR1
    • CD45 (PTPRC)
    • KRT8
    Please use search to find more...
  • Application Western Blotting (WB)
    • Western Blotting (WB)
    • ELISA
    • Immunohistochemistry (IHC)
    • Immunofluorescence (Paraffin-embedded Sections) (IF (p))
    • Immunofluorescence (IF)
    • Flow Cytometry (FACS)
    • Immunofluorescence (Cultured Cells) (IF (cc))
    • Immunocytochemistry (ICC)
    • Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    • Immunoprecipitation (IP)
    • Immunohistochemistry (Frozen Sections) (IHC (fro))
    • Enzyme Immunoassay (EIA)
    • Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))
    • Immunofluorescence (fixed cells) (IF/ICC)
    • Immunochromatography (IC)
    • Dot Blot (DB)
    • Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
    • Functional Studies (Func)
    • Radioimmunoassay (RIA)
    • Neutralization (Neut)
    • Chromatin Immunoprecipitation (ChIP)
    • Coating (Coat)
    • Staining Methods (StM)
    • Immunostaining (ISt)
    • Fluorescence Microscopy (FM)
    • Immunoassay (IA)
    • Immunodiffusion (ID)
    • Proximity Ligation Assay (PLA)
    • Lateral Flow (LF)
    • Surface Plasmon Resonance (SPR)
    • Confocal Microscopy (CM)
    • Immunoelectrophoresis (IEP)
    • Live Cell Imaging (LCI)
    • Bio-Layer Interferometry (BLI)
    • Cytotoxicity Test (CyTox)
    • Indirect Immunofluorescence Assay (IFA)
    • RNA Interference (RNAi)
    • Antibody Array (AA)
    • Blocking Antibody (Inhibition)
    • Blocking Reagent (BR)
    • Blocking Peptide (BP)
    • ELISA (Capture)
    • ChIP DNA-Sequencing (ChIP-seq)
    • In vivo Studies (in vivo)
    • Protein Assay (PrA)
    • Chemiluminescence Immunoassay (CLIA)
    • Affinity Purification (AP)
    • Cellular Assay (CA)
    • Intracellular Flow Cytometry (ICFC)
    • Luminex Assay (LMNX)
    Please use search to find more...
  • Reactivity
    • Human
    • Mouse
    • Rat
    • Cow
    • Dog
    • Rabbit
    • Horse
    • Guinea Pig
    • Pig
    • Monkey
    • Zebrafish (Danio rerio)
    • Chicken
    • Bat
    • Sheep
    • Hamster
    • Xenopus laevis
    • Goat
    • Saccharomyces cerevisiae
    • Drosophila melanogaster
    • Arabidopsis thaliana
    • Virus
    • E. coli
    • Cat
    • Various Species
    • Plant
    • Human Immunodeficiency Virus (HIV)
    • Mammalian
    • Frog
    • Fish
    • Bacteria
    • Hepatitis C Virus (HCV)
    • Chimpanzee
    • Aequorea victoria
    • Oryza sativa
    • Non-Human Primate
    • Primate
    • Rhesus Monkey
    • SARS Coronavirus-2 (SARS-CoV-2)
    • C. elegans
    • Chemical
    • Tag
    • Cynomolgus
    • Avian
    • Zea mays
    • Arabidopsis
    • Influenza A Virus
    • Hepatitis B Virus (HBV)
    • Schistosoma japonicum
    • Others
    • Staphylococcus aureus
    Please use search to find more...
  • Cited in publications
  • Images available
  • Independent Validation
  • Preservative free only
  • Carrier free only
  • Detection Method
    • Fluorometric
  • Host
    • Rabbit
    • Mouse
    • Goat
    • Sheep
    • Rat
    • Guinea Pig
    • Chicken
    • Human
    • Hamster
    • Alpaca
    • Mammalian Cells
    • Humanized
    • Chicken egg
    • Llama
    • Cell culture
    • Human Cells
    • Armenian Hamster
    • Cow
    • HEK-293F Cells
    • Chicken eggs
    • Horse
    • CHO Cells
    • Dog
    • Donkey
    • Golden Syrian Hamster
    • Carp
    • Fruit Fly (Drosophila melanogaster)
    • Phage Display
    • Pig
    • Tobacco (Nicotiana benthamiana)
  • Clonality
    • Polyclonal
    • Monoclonal
    • Chimeric
    • Bispecific
  • Clone
    • C1
    • C2
    • C3
    • C4
    • C5
    • C6
    • C10
    • C7
    • C11
    • D1
    • D4
    • C8
    • 1F6
    • 1D6
    • C12
    • D2
    • 2C4
    • 2B11
    • D3
    • 1F4
    • 1C4
    • 1D2
    • 3G1
    • C9
    • 1F10
    • 2D3
    • 1F3
    • 2E9
    • 2F11
    • 1A8
    • 1F11
    • 1A7
    • 1D4
    • 1E8
    • 1F8
    • 1G5
    • 3F8
    • 3G4
    • 1A5
    • 1B2
    • 1D8
    • 1G2
    • 1C1
    • 1E1
    • 2C1
    • 1A4
    • 2D7
    • 1A1
    • 1B4
    • 1B7
    Please use search to find more...
  • Binding Specificity
    • C-Term
    • N-Term
    • Internal Region
    • Middle Region
    • AA 101-200
    • Center
    • AA 1-100
    • AA 1-30
    • AA 51-150
    • AA 201-300
    • AA 151-250
    • AA 21-120
    • AA 301-400
    • AA 251-350
    • AA 1-50
    • AA 31-130
    • AA 401-500
    • AA 351-450
    • AA 121-220
    • AA 501-600
    • AA 131-230
    • AA 451-550
    • Extracellular
    • AA 1-80
    • AA 81-180
    • AA 61-160
    • AA 231-330
    • Extracellular Domain
    • AA 221-320
    • AA 41-140
    • AA 35-84
    • AA 71-120
    • AA 601-700
    • AA 161-260
    • Intracellular
    • AA 331-430
    • AA 51-100
    • AA 50-100
    • AA 181-280
    • AA 701-800
    • Cytosolic
    • AA 551-650
    • AA 1-300
    • AA 141-240
    • AA 1-110
    • AA 30-110
    • AA 1-200
    • AA 107-156
    • AA 251-300
    • AA 65-160
    Please use search to find more...
  • Conjugate
    • Un-conjugated
    • Biotin
    • FITC
    • HRP
    • PE
    • APC
    • Cy3
    • AbBy Fluor® 594
    • Cy5
    • AbBy Fluor® 350
    • AbBy Fluor® 488
    • AbBy Fluor® 555
    • AbBy Fluor® 647
    • Cy5.5
    • Cy7
    • AbBy Fluor® 750
    • AbBy Fluor® 680
    • Alexa Fluor 350
    • Alexa Fluor 405
    • Alexa Fluor 647
    • Alexa Fluor 750
    • Alexa Fluor 488
    • Alexa Fluor 594
    • Alexa Fluor 555
    • Alexa Fluor 680
    • CF®740
    • CF®488A
    • CF®405S
    • CF®568
    • CF®640R
    • CF®647
    • CF®594
    • PE-Cy5
    • PE-Cy7
    • PE-Cy5.5
    • PerCP
    • Atto 488
    • Atto 390
    • Atto 594
    • Alkaline Phosphatase (AP)
    • Peroxidase (POD)
    • Texas Red (TR)
    • DyLight 488
    • DyLight 405
    • DyLight 549
    • DyLight 680
    • DyLight 649
    • DyLight 800
    • CF®555
    • Rhodamine
    • His tag
  • Isotype
    • IgG
    • Ig Fraction
    • IgG1
    • IgG2a
    • IgG1 kappa
    • IgG2b
    • kappa
    • IgG2a kappa
    • IgG2b kappa
    • IgG2
    • IgM
    • IgY
    • IgG3
    • IgG kappa
    • IgM kappa
    • IgG3 kappa
    • IgG2c
    • IgG1 lambda
    • IgG Mix lambda
    • IgG2a lambda
    • IgG2c kappa
    • L234A
    • L235A
    • P329G
    • IgG2b lambda
    • IgG lambda
    • IgG4
    • IgG2 lambda
    • Ig kappa
    • IgG1/IgG2b
    • IgG2 kappa
    • lambda
    • IgA
    • IgA kappa
    • IgG Mix kappa
    • D265A
    • IgG1b kappa
    • IgG3 lambda
    • IgG IgA IgM Mix lambda
    • IgG4 kappa
    • IgM lambda
    • Ig
    • Ig Mix
    • IgG2c lambda
    • Fc Silenced
    • IgA2 kappa
    • IgE
    • IgE kappa
    • IgG IgA IgM Mix
    • IgG4 lambda
    Please use search to find more...
  • Protein Type
    • Recombinant Antibody
  • Format
    • Liquid
    • Lyophilized
    • Frozen
    • Lyophilized or Liquid
  • Fragment
    • single-domain Antibody (sdAb)
    • Fc fragment
    • Fab fragment
    • scFv fragment
    • F(ab')2 fragment
  • Grade
    • Verified
    • KD Validated
    • KO Validated
    • Carrier-free
    • Cell Culture Grade
    • In vivo Grade
    • Research Grade
    • ChIP-seq Grade
    • Animal-Free
    • GMP Grade
    • ChIP Grade
    • Calibrator Grade
    • MALS verified
    • Recent
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    • Assay Biotechnology Company, Inc.
    • Biomatik
    • Cloud-Clone
    • NSJ Bioreagents
    • FabGennix
    • RayBiotech
    • Rockland
    • Covalab
    • Angio-Proteomie
    • CUSABIO
    • Alomone Labs
    • Enogene Biotech
    • Antibodies Incorporated
    • Abiocode
    • OriGene
    • Wuhan Fine biotech
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    • Elabscience
    • Synaptic Systems
    • Abeomics
    • RevMAb Biosciences
    • Active Motif
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    Please use search to find more...
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