anti-Cow (Bovine) Perforin 1 antibody for Immunocytochemistry
Recommended Perforin 1 Antibody
- Antigen
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Perforin 1 (Pore Forming Protein) (PRF1) Antibodies
show synonyms for this antigen- FLH2
- HPLH2
- P1
- PFN1
- PFP
- PRF1
- Pfn
- Pfp
- Prf-1
- Cyta
- RATCYTA
- LOC443187
- perforin
- prf1
- cytolysin
- perforin-1
- perforin-1-like
- perforin 1
- perforin 1 (pore forming protein)
- perforin
- perforin 1 L homeolog
- PRF1
- Prf1
- LOC443187
- prf1
- prf1.L
- Reactivity
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Human, Cow (Bovine)
Alternatives - Host
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Mouse
Alternatives - Clonality
- Conjugate
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This Perforin 1 antibody is conjugated to PE
Alternatives - Application
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Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Alternatives - Options
Available images
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| Relevance Score | ABIN | Application | Conjugate | Host | Isotype | Epitope | Clonality | References | Details |
|---|---|---|---|---|---|---|---|---|---|
| 16.847717 | ABIN1981903 | IHC (f) ICC FACS IP IHC (p) | Mouse | IgG2b | dG9 | 3 | |||
| 1 | ABIN3200987 | FACS ICC IHC IP | PE | Mouse | IgG2b | dG9 | 0 | ||
| 1 | ABIN3200745 | FACS ICC IHC IP | Mouse | IgG2b | dG9 | 0 |
General |
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|---|---|
| Antigen | Perforin 1 (Pore Forming Protein) (PRF1) Antibodies |
| Reactivity | Human, Cow (Bovine) Alternatives |
| Host | Mouse Alternatives |
| Clonality (Clone) | |
| Conjugate | This Perforin 1 antibody is conjugated to PE Alternatives |
| Application |
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Alternatives
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3 references available |
| Supplier | |
Product Details anti-Perforin 1 AntibodyTarget Details Perforin 1 Application Details Handling References for anti-Perforin 1 antibody (ABIN1981897) Images |
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| Specificity | The mouse monoclonal antibody dG9 (also known as deltaG9) recognizes perforin, a 70 kDa protein expressed in cytoplasmic granules of cytotoxic T cells and NK cells. |
| No Cross-Reactivity | Mouse (Murine) |
| Cross-Reactivity (Details) | Human, Bovine |
| Immunogen | purified granules from human YT lymphoma cell line |
| Clone | DG9 |
| Isotype | IgG2b |
| Plasmids, Primers & others | |
Target Details Perforin 1Product Details anti-Perforin 1 Antibody Application Details Handling References for anti-Perforin 1 antibody (ABIN1981897) Images back to top |
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| Antigen | |
| Alternative Name | Perforin (PRF1 Antibody Abstract) |
| Background | Perforin is a 70 kDa cytolytic protein with structural and functional similarities to complement component 9 (C9). It is stored in cytoplasmic granules of cytotoxic T cells and NK cells and after its release it forms transmembrane pores in the target cells to kill it. As perforin is a key effector molecule for cell-mediated cytolysis, defects of its gene can cause severe disorders. |
| Gene ID | 5551 |
| Pathways | Apoptosis, Caspase Cascade in Apoptosis |
Application DetailsProduct Details anti-Perforin 1 Antibody Target Details Perforin 1 Handling References for anti-Perforin 1 antibody (ABIN1981897) Images back to top |
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| Application Notes | The reagent is designed for Flow Cytometry analysis of human blood cells using 10 μ,l reagent / 100 μ,l of whole blood or 106 cells in a suspension.The content of a vial (1 ml) is sufficient for 100 tests. |
| Comment |
The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary. |
| Restrictions | For Research Use only |
HandlingProduct Details anti-Perforin 1 Antibody Target Details Perforin 1 Application Details References for anti-Perforin 1 antibody (ABIN1981897) Images back to top |
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| Buffer | The reagent is provided in stabilizing phosphate buffered saline (PBS) solution containing 15 mM sodium azide. |
| Preservative | Sodium azide |
| Precaution of Use | This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Handling Advice |
Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label. Short-term exposure to room temperature should not affect the quality of the reagent. However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user. |
| Storage | 4 °C |
| Storage Comment | Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label. |
References for anti-Perforin 1 antibody (ABIN1981897)Product Details anti-Perforin 1 Antibody Target Details Perforin 1 Application Details Handling Images back to top |
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| Product cited in: |
Qiu, Chen, Liao, Zhang, Wang, Li, Luo, Fang, Li, Zhou, Shen, Chen, Huang, Cai, Cao, Jiang, Zeng, Chen: "Tim-3-expressing CD4+ and CD8+ T cells in human tuberculosis (TB) exhibit polarized effector memory phenotypes and stronger anti-TB effector functions." in: PLoS pathogens, Vol. 8, Issue 11, pp. e1002984, 2012 Takeuchi, Inoue, Otani, Yamasaki, Nakamura, Kibata: "Cell-in-cell structures formed between human cancer cell lines and the cytotoxic regulatory T-cell line HOZOT." in: Journal of molecular cell biology, Vol. 2, Issue 3, pp. 139-51, 2010 Hersperger, Makedonas, Betts: "Flow cytometric detection of perforin upregulation in human CD8 T cells." in: Cytometry. Part A : the journal of the International Society for Analytical Cytology, Vol. 73, Issue 11, pp. 1050-7, 2008 |
ImagesProduct Details anti-Perforin 1 Antibody Target Details Perforin 1 Application Details Handling References for anti-Perforin 1 antibody (ABIN1981897) back to top |
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| Supplier Images |
Intracellular staining of perforin in human peripheral blood cells with anti-perforin...
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