anti-Human Perforin 1 antibody for Flow Cytometry

Recommended Perforin 1 Antibody

Antigen
Perforin 1 (Pore Forming Protein) (PRF1) Antibodies
  • FLH2
  • HPLH2
  • P1
  • PFN1
  • PFP
  • PRF1
  • Pfn
  • Pfp
  • Prf-1
  • Cyta
  • RATCYTA
  • LOC443187
  • perforin
  • prf1
  • cytolysin
  • perforin-1
  • perforin-1-like
  • perforin 1
  • perforin 1 (pore forming protein)
  • perforin
  • perforin 1 L homeolog
  • PRF1
  • Prf1
  • LOC443187
  • prf1
  • prf1.L
Reactivity
Human, Cow (Bovine)
152
45
24
8
2
2
1
1
1
Host
Mouse
101
78
9
Clonality
Monoclonal
Conjugate
This Perforin 1 antibody is conjugated to PE
19
16
8
4
2
2
2
2
2
2
2
Application
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
103
70
45
41
36
18
18
17
15
13
8
6
4
2
1
1
1
1
1
1
1
Options

Available images

Catalog No. ABIN1981897
$ 484.24
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Clonality References Details
19.857311 ABIN1383925 FACS PE Mouse IgG1 B-D48 1
16.857311 ABIN1981903 IHC (f) ICC FACS IP IHC (p) Mouse IgG2b dG9 3
16.857311 ABIN1383924 FACS FITC Mouse IgG1 B-D48 1
16.857311 ABIN1383923 IHC (fro) FACS WB Mouse IgG1 B-D48 1
15.357312 ABIN1383927 FACS PerCP Mouse IgG1 B-D48 0
15.357312 ABIN1383926 FACS Mouse IgG1 B-D48 0
12.995888 ABIN2662106 FACS Pacific Blue Mouse IgG2b kappa dG9 10
12.995888 ABIN2662365 FACS Pacific Blue Mouse IgG1 B-D48 2
12.357312 ABIN934012 FACS IHC Biotin Mouse IgG2b delta G9 0
12.357312 ABIN289189 FACS IHC FITC Mouse IgG2b kappa delta G9 0
12.357312 ABIN289190 FACS IHC PE Mouse IgG2b kappa delta G9 0
12.357312 ABIN284673 FACS IHC Mouse IgG2b delta G9 0
12.357312 ABIN2148785 FACS IF IP IHC Mouse IgG2b DG9 0
12.357312 ABIN2148783 Func FACS IP IHC Mouse IgG2b BOR21 0
9.995888 ABIN2657572 FACS Alexa Fluor 488 Mouse IgG2b kappa dG9 11
9.995888 ABIN2657991 FACS Alexa Fluor 647 Mouse IgG2b kappa dG9 11
9.995888 ABIN2661985 FACS FITC Mouse IgG2b kappa dG9 10
9.995888 ABIN2658819 FACS APC Mouse IgG2b kappa dG9 10
9.995888 ABIN2665326 FACS Mouse IgG2b kappa dG9 10
9.995888 ABIN3039419 FACS IF/ICC IHC IP PE Mouse IgG2b 0

General

Antigen Perforin 1 (Pore Forming Protein) (PRF1) Antibodies
Reactivity Human, Cow (Bovine)
(152), (45), (24), (8), (2), (2), (1), (1), (1)
Host Mouse
(101), (78), (9)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Perforin 1 antibody is conjugated to PE
(19), (16), (8), (4), (2), (2), (2), (2), (2), (2), (2)
Application Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(103), (70), (45), (41), (36), (18), (18), (17), (15), (13), (8), (6), (4), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 3 references available
Supplier

Product Details anti-Perforin 1 Antibody

Target Details Perforin 1 Application Details Handling References for anti-Perforin 1 antibody (ABIN1981897) Images
Specificity The mouse monoclonal antibody dG9 (also known as deltaG9) recognizes perforin, a 70 kDa protein expressed in cytoplasmic granules of cytotoxic T cells and NK cells.
No Cross-Reactivity Mouse (Murine)
Cross-Reactivity (Details) Human, Bovine
Immunogen purified granules from human YT lymphoma cell line
Clone DG9
Isotype IgG2b
Plasmids, Primers & others

Target Details Perforin 1

Product Details anti-Perforin 1 Antibody Application Details Handling References for anti-Perforin 1 antibody (ABIN1981897) Images back to top
Antigen
Alternative Name Perforin (PRF1 Antibody Abstract)
Background Perforin is a 70 kDa cytolytic protein with structural and functional similarities to complement component 9 (C9). It is stored in cytoplasmic granules of cytotoxic T cells and NK cells and after its release it forms transmembrane pores in the target cells to kill it. As perforin is a key effector molecule for cell-mediated cytolysis, defects of its gene can cause severe disorders.
Gene ID 5551
Pathways Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-Perforin 1 Antibody Target Details Perforin 1 Handling References for anti-Perforin 1 antibody (ABIN1981897) Images back to top
Application Notes The reagent is designed for Flow Cytometry analysis of human blood cells using 10 μ,l reagent / 100 μ,l of whole blood or 106 cells in a suspension.The content of a vial (1 ml) is sufficient for 100 tests.
Comment

The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

Restrictions For Research Use only

Handling

Product Details anti-Perforin 1 Antibody Target Details Perforin 1 Application Details References for anti-Perforin 1 antibody (ABIN1981897) Images back to top
Buffer The reagent is provided in stabilizing phosphate buffered saline (PBS) solution containing 15 mM sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze. Avoid prolonged exposure to light.
Do not use after expiration date stamped on vial label.
Short-term exposure to room temperature should not affect the quality of the reagent. However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.
Storage 4 °C
Storage Comment Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.

References for anti-Perforin 1 antibody (ABIN1981897)

Product Details anti-Perforin 1 Antibody Target Details Perforin 1 Application Details Handling Images back to top
Product cited in:

Qiu, Chen, Liao, Zhang, Wang, Li, Luo, Fang, Li, Zhou, Shen, Chen, Huang, Cai, Cao, Jiang, Zeng, Chen: "Tim-3-expressing CD4+ and CD8+ T cells in human tuberculosis (TB) exhibit polarized effector memory phenotypes and stronger anti-TB effector functions." in: PLoS pathogens, Vol. 8, Issue 11, pp. e1002984, 2012

Takeuchi, Inoue, Otani, Yamasaki, Nakamura, Kibata: "Cell-in-cell structures formed between human cancer cell lines and the cytotoxic regulatory T-cell line HOZOT." in: Journal of molecular cell biology, Vol. 2, Issue 3, pp. 139-51, 2010

Hersperger, Makedonas, Betts: "Flow cytometric detection of perforin upregulation in human CD8 T cells." in: Cytometry. Part A : the journal of the International Society for Analytical Cytology, Vol. 73, Issue 11, pp. 1050-7, 2008

Images

Product Details anti-Perforin 1 Antibody Target Details Perforin 1 Application Details Handling References for anti-Perforin 1 antibody (ABIN1981897) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-Perforin 1 (Pore Forming Protein) (PRF1) antibody (PE) (ABIN1981897) Intracellular staining of perforin in human peripheral blood cells with anti-perforin...