anti-Human Perforin 1 antibody for Western Blotting

Recommended Perforin 1 Antibody

Antigen
Perforin 1 (Pore Forming Protein) (PRF1) Antibodies
  • FLH2
  • HPLH2
  • P1
  • PFN1
  • PFP
  • PRF1
  • Pfn
  • Pfp
  • Prf-1
  • Cyta
  • RATCYTA
  • LOC443187
  • perforin
  • prf1
  • cytolysin
  • perforin-1
  • perforin-1-like
  • perforin 1
  • perforin 1 (pore forming protein)
  • perforin
  • perforin 1 L homeolog
  • PRF1
  • Prf1
  • LOC443187
  • prf1
  • prf1.L
Reactivity
Mouse (Murine), Rat (Rattus), Human
149
44
23
9
2
2
1
1
1
Host
Rabbit
100
76
9
Clonality
Polyclonal
Conjugate
This Perforin 1 antibody is un-conjugated
19
16
9
4
2
2
2
2
2
2
2
Application
Immunohistochemistry (IHC), Western Blotting (WB)
101
68
42
41
37
19
18
17
16
13
8
6
2
2
2
1
1
1
1
1
1
Options

Available images

Catalog No. ABIN3020642
$ 393.25
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Clonality References Details
17.30899 ABIN1383923 IHC (fro) FACS WB Mouse IgG1 B-D48 1
16.609173 ABIN2854389 WB Rabbit IgG N-Term Polyclonal 0
16.609173 ABIN634500 WB Rabbit Polyclonal 0
12.808991 ABIN4902121 ELISA WB Rabbit IgG AA 451-500 Polyclonal 0
12.808991 ABIN2149646 ELISA WB Rabbit IgG Polyclonal 0
12.808991 ABIN5623306 WB Rabbit IgG Polyclonal 0
10.800182 ABIN519155 IP ELISA WB Mouse IgG2a kappa AA 461-555 3B4 0
10.800182 ABIN3032180 WB Rabbit IgG AA 175-555 Polyclonal 0
7.8001823 ABIN3222786 IHC WB Rabbit C-Term Polyclonal 0
7.8001823 ABIN3043547 WB Rabbit IgG AA 175-555 Polyclonal 0
7.8001823 ABIN3197764 IC IF WB Rabbit Polyclonal 0
7.8001823 ABIN6264417 ELISA IHC WB Rabbit IgG Polyclonal 0
7.8001823 ABIN6671994 IHC WB Rabbit IgG Polyclonal 0
7.8001823 ABIN1874263 IF IHC WB Rabbit IgG Polyclonal 0
7.8001823 ABIN948310 IP WB Rabbit AA 1-555 Polyclonal 0
7.8001823 ABIN2988384 IF/ICC IHC WB Rabbit IgG Polyclonal 0
7.8001823 ABIN6005988 IF/ICC WB Rabbit IgG full length Polyclonal 0
7.8001823 ABIN2919582 IF/ICC IHC IP WB Rabbit AA 32-316 Polyclonal 0
7 ABIN187563 IP WB Mouse IgG2a AA 402-534 CE2-10 0
4.8001823 ABIN2775294 WB Rabbit N-Term Polyclonal 1

General

Antigen Perforin 1 (Pore Forming Protein) (PRF1) Antibodies
Reactivity Mouse (Murine), Rat (Rattus), Human
(149), (44), (23), (9), (2), (2), (1), (1), (1)
Host Rabbit
(100), (76), (9)
Clonality
Polyclonal
Conjugate This Perforin 1 antibody is un-conjugated
(19), (16), (9), (4), (2), (2), (2), (2), (2), (2), (2)
Application Immunohistochemistry (IHC), Western Blotting (WB)
(101), (68), (42), (41), (37), (19), (18), (17), (16), (13), (8), (6), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Supplier

Product Details anti-Perforin 1 Antibody

Target Details Perforin 1 Application Details Handling Images
Purification Affinity purification
Immunogen Recombinant protein of human PRF1
Isotype IgG
Plasmids, Primers & others

Target Details Perforin 1

Product Details anti-Perforin 1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name PRF1 (PRF1 Antibody Abstract)
Background Granzymes are a family of serine proteases expressed by cytotoxic T lymphocytes and natural killer (NK) cells and are key components of immune responses to pathogens and transformed cells. Granzymes are synthesized as zymogens and are processed into mature enzymes by cleavage of a leader sequence. They are released by exocytosis in lysosome-like granules containing perforin, a membrane pore-forming protein. Granzyme B has the strongest apoptotic activity of all the granzymes as a result of its caspase-like ability to cleave substrates at aspartic acid residues thereby activating procaspases directly and cleaving downstream caspase substrates. Perforin is a pore-forming protein that facilitates the entry of cytotoxic serine proteases, such as granzymes, into target cells. Perforin is primarily expressed in cytotoxic T lymphocytes and NK cells.Ma, L. and Pei, G. J Cell Sci 120, 213-8.  
Synonyms: PRF1, FLH2, HPLH2, MGC65093, P1, PFN1, PFP
Gene ID 5551
UniProt P14222
Pathways Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-Perforin 1 Antibody Target Details Perforin 1 Handling Images back to top
Application Notes Recommended dilutions: WB: 1:500-1:2000, IHC: 1:50-1:200
Restrictions For Research Use only

Handling

Product Details anti-Perforin 1 Antibody Target Details Perforin 1 Application Details Images back to top
Format Liquid
Buffer PBS with 0.02 % sodium azide, 50 % glycerol, pH 7.3
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze / thaw cycles
Storage -20 °C/-80 °C

Images

Product Details anti-Perforin 1 Antibody Target Details Perforin 1 Application Details Handling back to top
Supplier Images
Image no. 1 for anti-Perforin 1 (Pore Forming Protein) (PRF1) antibody (ABIN3020642) Western blot analysis of extracts of various cell lines, using PRF1 antibody.
Image no. 2 for anti-Perforin 1 (Pore Forming Protein) (PRF1) antibody (ABIN3020642) Immunofluorescence analysis of HeLa cell using PRF1 antibody. Blue: DAPI for nuclear ...