anti-Mouse (Murine) Perforin 1 antibody for Western Blotting

Recommended Perforin 1 Antibody

Antigen
Perforin 1 (Pore Forming Protein) (PRF1) Antibodies
  • FLH2
  • HPLH2
  • P1
  • PFN1
  • PFP
  • PRF1
  • Pfn
  • Pfp
  • Prf-1
  • Cyta
  • RATCYTA
  • LOC443187
  • perforin
  • prf1
  • cytolysin
  • perforin-1
  • perforin-1-like
  • perforin 1
  • perforin 1 (pore forming protein)
  • perforin
  • perforin 1 L homeolog
  • PRF1
  • Prf1
  • LOC443187
  • prf1
  • prf1.L
Reactivity
Mouse (Murine), Rat (Rattus), Human
149
44
23
9
2
2
1
1
1
Host
Rabbit
100
76
9
Clonality
Polyclonal
Conjugate
This Perforin 1 antibody is un-conjugated
19
16
9
4
2
2
2
2
2
2
2
Application
Immunohistochemistry (IHC), Western Blotting (WB)
101
68
42
41
37
19
18
17
16
13
8
6
2
2
2
1
1
1
1
1
1
Options

Available images

Catalog No. ABIN3020642
$ 393.25
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Clonality References Details
10 ABIN187561 IHC (fro) IF IHC (p) WB Rat IgG2a CB5-4 0
7.8001823 ABIN3197764 IC IF WB Rabbit Polyclonal 0
7.8001823 ABIN6264417 ELISA IHC WB Rabbit IgG Polyclonal 0
7.8001823 ABIN6671994 IHC WB Rabbit IgG Polyclonal 0
7.8001823 ABIN2988384 IF/ICC IHC WB Rabbit IgG Polyclonal 0
7.8001823 ABIN6005988 IF/ICC WB Rabbit IgG full length Polyclonal 0
7.8001823 ABIN2919583 IF/ICC IHC IP WB Rabbit AA 40-355 Polyclonal 0
7 ABIN187563 IP WB Mouse IgG2a AA 402-534 CE2-10 0
4.8001823 ABIN411557 IF IP IHC WB Rabbit IgG Polyclonal 0
4.8001823 ABIN1860312 ICC IHC WB Rabbit IgG AA 40-355 Polyclonal 0
4 ABIN535345 IHC (fro) IF IHC (p) IP WB Rat IgG2a CB5-4 0
1 ABIN6757074 WB Rabbit IgG Polyclonal 0
1 ABIN2576859 WB Rabbit IgG AA 40-355 Polyclonal 0
1 ABIN199825 ICC IHC IHC (fro) IHC (p) IP WB Rat IgG2a AA 98-534 CB5-4 0
1 ABIN6803119 WB Biotin Rabbit IgG AA 40-355 Polyclonal 0
1 ABIN6808290 WB FITC Rabbit IgG AA 40-355 Polyclonal 0
1 ABIN603642 WB Rabbit IgG Polyclonal 0
1 ABIN6758182 IF IHC WB Rabbit IgG Polyclonal 0
1 ABIN2333254 IC IP IHC WB Rat IgG2a AA 98-534 6D72 0
1 ABIN2333273 IC IHC ELISA WB Rabbit IgG AA 40-355 Polyclonal 0

General

Antigen Perforin 1 (Pore Forming Protein) (PRF1) Antibodies
Reactivity Mouse (Murine), Rat (Rattus), Human
(149), (44), (23), (9), (2), (2), (1), (1), (1)
Host Rabbit
(100), (76), (9)
Clonality
Polyclonal
Conjugate This Perforin 1 antibody is un-conjugated
(19), (16), (9), (4), (2), (2), (2), (2), (2), (2), (2)
Application Immunohistochemistry (IHC), Western Blotting (WB)
(101), (68), (42), (41), (37), (19), (18), (17), (16), (13), (8), (6), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Supplier

Product Details anti-Perforin 1 Antibody

Target Details Perforin 1 Application Details Handling Images
Purification Affinity purification
Immunogen Recombinant protein of human PRF1
Isotype IgG
Plasmids, Primers & others

Target Details Perforin 1

Product Details anti-Perforin 1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name PRF1 (PRF1 Antibody Abstract)
Background Granzymes are a family of serine proteases expressed by cytotoxic T lymphocytes and natural killer (NK) cells and are key components of immune responses to pathogens and transformed cells. Granzymes are synthesized as zymogens and are processed into mature enzymes by cleavage of a leader sequence. They are released by exocytosis in lysosome-like granules containing perforin, a membrane pore-forming protein. Granzyme B has the strongest apoptotic activity of all the granzymes as a result of its caspase-like ability to cleave substrates at aspartic acid residues thereby activating procaspases directly and cleaving downstream caspase substrates. Perforin is a pore-forming protein that facilitates the entry of cytotoxic serine proteases, such as granzymes, into target cells. Perforin is primarily expressed in cytotoxic T lymphocytes and NK cells.Ma, L. and Pei, G. J Cell Sci 120, 213-8.  
Synonyms: PRF1, FLH2, HPLH2, MGC65093, P1, PFN1, PFP
Gene ID 5551
UniProt P14222
Pathways Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-Perforin 1 Antibody Target Details Perforin 1 Handling Images back to top
Application Notes Recommended dilutions: WB: 1:500-1:2000, IHC: 1:50-1:200
Restrictions For Research Use only

Handling

Product Details anti-Perforin 1 Antibody Target Details Perforin 1 Application Details Images back to top
Format Liquid
Buffer PBS with 0.02 % sodium azide, 50 % glycerol, pH 7.3
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze / thaw cycles
Storage -20 °C/-80 °C

Images

Product Details anti-Perforin 1 Antibody Target Details Perforin 1 Application Details Handling back to top
Supplier Images
Image no. 1 for anti-Perforin 1 (Pore Forming Protein) (PRF1) antibody (ABIN3020642) Western blot analysis of extracts of various cell lines, using PRF1 antibody.
Image no. 2 for anti-Perforin 1 (Pore Forming Protein) (PRF1) antibody (ABIN3020642) Immunofluorescence analysis of HeLa cell using PRF1 antibody. Blue: DAPI for nuclear ...