Atpalpha antibody (Internal Region)
This Rabbit Polyclonal antibody specifically detects Atpalpha in IHC, IHC (p) and WB. It exhibits reactivity toward Drosophila melanogaster and Human.
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Catalog No. ABIN1493677
Quick Overview for Atpalpha antibody (Internal Region) (ABIN1493677)
Target
Atpalpha (ATPALPHA)
(Na Pump alpha Subunit (ATPALPHA))
Reactivity
Drosophila melanogaster, Human
Host
Rabbit
Clonality
Polyclonal
Application
Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Binding Specificity
- Internal Region
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Specificity
- Drosophila, human Atpalpha
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Predicted Reactivity
- Percent identity by BLAST analysis:
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Purification
- Immunoaffinity purified
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Immunogen
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Recombinant fragment corresponding to a region within amino acids 317 and 569 of Atpalpha (SwissProt P13607). Percent identity by BLAST analysis:
Type of Immunogen: Recombinant protein -
Isotype
- IgG
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Application Notes
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Approved: IHC, IHC-P (1:100 - 1:1000), WB (1:5000 - 1:20000)
Usage: Suggested starting dilutions are as follows: Western blot: 1:5000-1:20000, IHC-Paraffin: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10 mM Citrate buffer ( pH 6.0) or Tris-EDTA buffer ( pH 8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. -
Comment
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Target Species of Antibody: Drosophila
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- Lot specific
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Buffer
- PBS, pH 7, 1 % BSA, 20 % Glycerol, 0.01 % Thimerosal
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Preservative
- Thimerosal (Merthiolate)
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Precaution of Use
- This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handling Advice
- Avoid freeze-thaw cycles.
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Storage
- -20 °C
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Storage Comment
- Aliquot and freeze at -20°C. Avoid freeze-thaw cycles.
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- Atpalpha (ATPALPHA) (Na Pump alpha Subunit (ATPALPHA))
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Alternative Name
- Atpalpha
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Background
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Name/Gene ID: Atpalpha
Synonyms: Atpalpha, Atp a, Atpa, ATPBagr, CG5670, Da-47, DnATPase, Dmel\CG5670, K[+]-ATPase, K ATPase, L(3)04492, L(3)j6A4, L(3)rM454, Na+-K+-ATPase, Na/K-ATPase alpha-subunit, Na[+], Na[+]K[+]ATPase, Na[+]K[+] ATPase, NaK ATPase, JY, K-ATPase, K[+]-ATPase alpha subunit, L(3)01164, L(3)j3B7, Na+/K+ ATPase alpha subunit, Na-P, NAK, K[+] -ATPase, K[+]-ATPase alpha-subunit, L(3)01453, L(3)j3B2, L(3)ry136, Na pump alpha subunit, Na[+]/K[+] ATPase, Na[+]/K[+]ATPase -
Gene ID
- 48971
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UniProt
- P13607
Target
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