High Affinity Ni-Charged Resin

Details for Product No. ABIN2017616
Application
Purification (Purif)
Options
Specificity High binding capacity: The kit can handle over 20 mg 6xHis-tagged protein/mL (CV).
Simple purification procedure under both native and denaturing conditions.
Broad pH stability: The resin works well between pH 3 and pH 13, and can manage pH 2 and pH 14 for short periods.
Resilient: If properly regenerated, the resin can be reused multiple times.
Characteristics High Affinity Ni-Charged Resin is an 4 % cross-linked agarose medium covalently coupled to a chelating agent that binds Ni2+ by four coordination sites for high-affinity purification of polyhistidine-tagged recombinant proteins. High Affinity Ni-Charged Resin has low Ni2+ leakage, high protein-binding capacity and stability, and is compatible with a wide range of additives used in protein purification. This makes High Affinity Ni-Charged Resin the excellent choice for high performance purification of polyhistidine-tagged proteins. High Affinity Ni-Charged Resin is available in 10, 25 and 500 mL lab packs.
Total Volume: 10 mL settled resin (20 mL 50 % slurry) 25 mL settled resin (50 mL 50 % slurry) 500 mL settled resin (1000 mL 50 % slurry)
Bead Ligand Nickel ions
Bead Matrix Agarose beads
Bead Size 90 µm
Comment

For ambient pressure use only.

Restrictions For Research Use only
Format Liquid
Storage 4 °C
Storage Comment Store the product between 2°C and 8°C.
Product cited in: Morgado, Zeth, Burmann, Maier, Hiller: "Characterization of the insertase BamA in three different membrane mimetics by solution NMR spectroscopy." in: Journal of biomolecular NMR, Vol. 61, Issue 3-4, pp. 333-45, 2015 (PubMed).

Albert, Schlesinger, Viall, Mulla, Brosens, Chamley, Abrahams: "Effect of hydroxychloroquine on antiphospholipid antibody-induced changes in first trimester trophoblast function." in: American journal of reproductive immunology (New York, N.Y. : 1989), Vol. 71, Issue 2, pp. 154-64, 2014 (PubMed).

Zhang, Song, Cheng, Hao, Wang, Kan, Jin, Yu: "The acid phosphatase-encoding gene GmACP1 contributes to soybean tolerance to low-phosphorus stress." in: PLoS genetics, Vol. 10, Issue 1, pp. e1004061, 2014 (PubMed).

Jiang, Zhang, Zhao, Jia, Zhao, Wang: "A new group of anti-lipopolysaccharide factors from Marsupenaeus japonicus functions in antibacterial response." in: Developmental and comparative immunology, Vol. 48, Issue 1, pp. 33-42, 2014 (PubMed).

Jiang, Sun, Wang, Zhao, Wang: "A single whey acidic protein domain containing protein (SWD) inhibits bacteria invasion and dissemination in shrimp Marsupenaeus japonicus." in: Fish & shellfish immunology, Vol. 35, Issue 2, pp. 310-8, 2013 (PubMed).

Chai, Yu, Liu, Zhu: "A fibrinogen-related protein (TfFREP2) gene involving in the immune response of Trachidermus fasciatus against Vibrio anguillarum." in: Fish & shellfish immunology, Vol. 35, Issue 6, pp. 1988-92, 2013 (PubMed).

Sun, Zheng, Wang, Li, Shen: "Apoptotic effect of Vibrio vulnificus cytolysin on A549 human lung adenocarcinoma cells." in: Molecular medicine reports, Vol. 5, Issue 3, pp. 668-74, 2012 (PubMed).

Worzfeld, Swiercz, Looso, Straub, Sivaraj, Offermanns: "ErbB-2 signals through Plexin-B1 to promote breast cancer metastasis." in: The Journal of clinical investigation, Vol. 122, Issue 4, pp. 1296-305, 2012 (PubMed).

Zhao, Lv, Zhao, Huang, Xiao: "Expression of human globular adiponectin-glucagon-like peptide-1 analog fusion protein and its assay of glucose-lowering effect in vivo." in: International journal of medical sciences, Vol. 8, Issue 3, pp. 203-9, 2011 (PubMed).

Wu, Liu, Guo, Zhang, Zhao, Yang, Liu: "A monoclonal antibody specific to human myxovirus resistance protein A." in: Hybridoma (2005), Vol. 29, Issue 1, pp. 31-6, 2010 (PubMed).

Song, Liu, Wang, Zhang, You, Wang, Liu, Sun: "Vaccination with combination of Fit3L and RANTES in a DNA prime-protein boost regimen elicits strong cell-mediated immunity and antitumor effect." in: Vaccine, Vol. 27, Issue 7, pp. 1111-8, 2009 (PubMed).

Zhao, Sun, Ruan, Zhao, Lu, Yan: "Vibrio vulnificus cytolysin induces apoptosis in HUVEC, SGC-7901 and SMMC-7721 cells via caspase-9/3-dependent pathway." in: Microbial pathogenesis, Vol. 46, Issue 4, pp. 194-200, 2009 (PubMed).