High Affinity Ni-Charged Resin Bead
- Purification (Purif)
- High binding capacity: The kit can handle over 20 mg 6xHis-tagged protein/mL (CV).
Simple purification procedure under both native and denaturing conditions.
Broad pH stability: The resin works well between pH 3 and pH 13, and can manage pH 2 and pH 14 for short periods.
Resilient: If properly regenerated, the resin can be reused multiple times.
- High Affinity Ni-Charged Resin is an 4 % cross-linked agarose medium covalently coupled to a chelating agent that binds Ni2+ by four coordination sites for high-affinity purification of polyhistidine-tagged recombinant proteins. High Affinity Ni-Charged Resin has low Ni2+ leakage, high protein-binding capacity and stability, and is compatible with a wide range of additives used in protein purification. This makes High Affinity Ni-Charged Resin the excellent choice for high performance purification of polyhistidine-tagged proteins. High Affinity Ni-Charged Resin is available in 10, 25 and 500 mL lab packs.
Total Volume: 10 mL settled resin (20 mL 50 % slurry) 25 mL settled resin (50 mL 50 % slurry) 500 mL settled resin (1000 mL 50 % slurry)
- Bead Ligand
- Nickel ions
- Bead Matrix
- Agarose beads
- Bead Size
- 90 µm
For ambient pressure use only.
- For Research Use only
- 4 °C
- Storage Comment
- Store the product between 2°C and 8°C.
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