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High Affinity Ni-Charged Resin Bead

Purif Nickel ions Agarose beads 90 µm
Pubmed (12 references)
Catalog No. ABIN2017616
Plus shipping costs $45.00
10 mL
local_shipping Shipping to: United States
Delivery in 2 to 3 Business Days
  • Application
    Purification (Purif)
    High binding capacity: The kit can handle over 20 mg 6xHis-tagged protein/mL (CV).
    Simple purification procedure under both native and denaturing conditions.
    Broad pH stability: The resin works well between pH 3 and pH 13, and can manage pH 2 and pH 14 for short periods.
    Resilient: If properly regenerated, the resin can be reused multiple times.
    High Affinity Ni-Charged Resin is an 4 % cross-linked agarose medium covalently coupled to a chelating agent that binds Ni2+ by four coordination sites for high-affinity purification of polyhistidine-tagged recombinant proteins. High Affinity Ni-Charged Resin has low Ni2+ leakage, high protein-binding capacity and stability, and is compatible with a wide range of additives used in protein purification. This makes High Affinity Ni-Charged Resin the excellent choice for high performance purification of polyhistidine-tagged proteins. High Affinity Ni-Charged Resin is available in 10, 25 and 500 mL lab packs.
    Total Volume: 10 mL settled resin (20 mL 50 % slurry) 25 mL settled resin (50 mL 50 % slurry) 500 mL settled resin (1000 mL 50 % slurry)
    Bead Ligand
    Nickel ions
    Bead Matrix
    Agarose beads
    Bead Size
    90 µm
  • Comment

    For ambient pressure use only.

    For Research Use only
  • Format
    4 °C
    Storage Comment
    Store the product between 2°C and 8°C.
  • Morgado, Zeth, Burmann, Maier, Hiller: "Characterization of the insertase BamA in three different membrane mimetics by solution NMR spectroscopy." in: Journal of biomolecular NMR, Vol. 61, Issue 3-4, pp. 333-45, (2015) (PubMed).

    Albert, Schlesinger, Viall, Mulla, Brosens, Chamley, Abrahams: "Effect of hydroxychloroquine on antiphospholipid antibody-induced changes in first trimester trophoblast function." in: American journal of reproductive immunology (New York, N.Y. : 1989), Vol. 71, Issue 2, pp. 154-64, (2014) (PubMed).

    Zhang, Song, Cheng, Hao, Wang, Kan, Jin, Yu: "The acid phosphatase-encoding gene GmACP1 contributes to soybean tolerance to low-phosphorus stress." in: PLoS genetics, Vol. 10, Issue 1, pp. e1004061, (2014) (PubMed).

    Jiang, Zhang, Zhao, Jia, Zhao, Wang: "A new group of anti-lipopolysaccharide factors from Marsupenaeus japonicus functions in antibacterial response." in: Developmental and comparative immunology, Vol. 48, Issue 1, pp. 33-42, (2014) (PubMed).

    Jiang, Sun, Wang, Zhao, Wang: "A single whey acidic protein domain containing protein (SWD) inhibits bacteria invasion and dissemination in shrimp Marsupenaeus japonicus." in: Fish & shellfish immunology, Vol. 35, Issue 2, pp. 310-8, (2013) (PubMed).

    Chai, Yu, Liu, Zhu: "A fibrinogen-related protein (TfFREP2) gene involving in the immune response of Trachidermus fasciatus against Vibrio anguillarum." in: Fish & shellfish immunology, Vol. 35, Issue 6, pp. 1988-92, (2013) (PubMed).

    Sun, Zheng, Wang, Li, Shen: "Apoptotic effect of Vibrio vulnificus cytolysin on A549 human lung adenocarcinoma cells." in: Molecular medicine reports, Vol. 5, Issue 3, pp. 668-74, (2012) (PubMed).

    Worzfeld, Swiercz, Looso, Straub, Sivaraj, Offermanns: "ErbB-2 signals through Plexin-B1 to promote breast cancer metastasis." in: The Journal of clinical investigation, Vol. 122, Issue 4, pp. 1296-305, (2012) (PubMed).

    Zhao, Lv, Zhao, Huang, Xiao: "Expression of human globular adiponectin-glucagon-like peptide-1 analog fusion protein and its assay of glucose-lowering effect in vivo." in: International journal of medical sciences, Vol. 8, Issue 3, pp. 203-9, (2011) (PubMed).

    Wu, Liu, Guo, Zhang, Zhao, Yang, Liu: "A monoclonal antibody specific to human myxovirus resistance protein A." in: Hybridoma (2005), Vol. 29, Issue 1, pp. 31-6, (2010) (PubMed).

    Song, Liu, Wang, Zhang, You, Wang, Liu, Sun: "Vaccination with combination of Fit3L and RANTES in a DNA prime-protein boost regimen elicits strong cell-mediated immunity and antitumor effect." in: Vaccine, Vol. 27, Issue 7, pp. 1111-8, (2009) (PubMed).

    Zhao, Sun, Ruan, Zhao, Lu, Yan: "Vibrio vulnificus cytolysin induces apoptosis in HUVEC, SGC-7901 and SMMC-7721 cells via caspase-9/3-dependent pathway." in: Microbial pathogenesis, Vol. 46, Issue 4, pp. 194-200, (2009) (PubMed).

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