ALFA Catcher ST

Catalog No. ABIN7272858

Quick Overview for ALFA Catcher ST (ABIN7272858)

Target: ALFA-Tag

Application: Chromatin Immunoprecipitation (ChIP), Immunoprecipitation (IP), Protein Complex Immunoprecipitation (Co-IP)

Bead Ligand: Antibody

Bead Matrix: Agarose beads

Bead Size: 90 μm

Product Details

Purpose: ALFA Catcher ST (for “Super Tight”) is an innovative, high-affinity resin (Agarose) designed for efficient and clean pull-down of ALFA-tagged proteins.

Specificity: Recognizes the ALFA-tag (SRLEEELRRRLTE)

Characteristics: ALFA CatcherST is based on a single-domain antibody (sdAb), which is covalently immobilized on 4% cross-linked agarose or agarose-magnetic beads and binds ALFA-tagged proteins with extraordinarily high affinity (Kd = 26 pM). Target proteins are efficiently released from ALFA Catcher ST under acidic (e.g., 0.1 M glycin pH2.2) or denaturing conditions. The extremely tight interaction with ALFA-tagged proteins makes competitive elution using ALFA elution peptide inefficient. We recommend using ALFA Catcher PE for competitive elution under physiological and room temperature condition conditions. For efficient elutions at 4°C, we recommend using the ALFA Catcher CE

The innovative, oriented and highly selective attachment of the active sdAbs via a flexible linker guarantees optimal accessibility for target proteins and at the same time largely eliminates batch-to-batch variations. Due to the single-chain nature of sdAbs and their covalent attachment, no “leakage” of light and heavy chains from IgGs is observed even during elution with strongly denaturing and reducing conditions (e.g., SDS sample buffer). ALFA Catcher ST thus features high affinity and superior capacity for ALFA fusion proteins while showing negligible non-specific background.

Components: 4 % cross-linked agarose (bead size 50-150 μm) with covalently immobilized single-domain antibody

Material not included: wash buffers, columns, tubes

Application Details

Application Notes: Coating: sdAb anti-ALFA tag clone 1G5
Matrix: 4 % cross-linked agarose, bead size 50-150 μm
Capacity: > 3 µg ALFA-tagged GFP per µL of packed beads (= 2 μL of slurry)
Buffer Compatibility:

• Common buffer substances at pH 5 to 10
• 1 % Triton X-100, > 1% DDM, > 1 % CHAPS, 0.1 % SDS
• > 3 M NaCl, > 1 M MgSO4
• 6 M urea, > 2 M Guanidin-HCl
• > 100 mM DTT

Comment:

ALFA Catcher ST is compatible not only with physiological buffers but also with high stringency buffers. ALFA Catcher ST thus provides great freedom to adjust the binding and washing conditions to the experimental needs.

Restrictions: For Research Use only

Handling

Buffer: Resin is 50 % slurry in PBS containing 20 % Ethanol.

Storage: 4 °C

Storage Comment: Store at 4°C for up to 12 months. Do not freeze!

Expiry Date: 12 months

Target Details

Target: ALFA-Tag

Target Type: Tag

Background: The ALFA-tag is a novel, highly versatile epitope tag (core sequence SRLEEELRRRLTE) that can be employed for a wide range of life science applications.