BlockOut® Universal Blocking Buffer for Western Blotting

Catalog No. ABIN5706396

Supplier Product No.: mb-073

Quick Overview for BlockOut® Universal Blocking Buffer for Western Blotting (ABIN5706396)

Application: Western Blotting (WB)

Product Details

Supplier Product No.: mb-073

Supplier: Rockland

Purpose: BlockOut® Universal Blocking Buffer for Western Blotting

Brand: BlockOut®

Purification: BlockOut® Blocking buffer was prepared using ultra pure reagents dissolved in pharmaceutical grade water (WFI) and consists of a proprietary protein formulation in TRIS buffered saline at pH 7.6 with thimerosal added as an antimicrobial agent.

Sterility: Sterile filtered

Application Details

Application Notes:

Application Note: BlockOut® allows for superior signal detection and lower background noise in colorimetric, chemiluminescent, and fluorescent western blotting and other applications making it a convenient general purpose blocking agent. Antibody conjugates prepared with

Western Blot Dilution: User Defined

Other: Optimum performance is achieved using this product undiluted. However, this blocking solution can often be diluted 1:1 in TBS without a significant loss of performance.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 X

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: DO NOT FREEZE.

Storage: 4 °C

Storage Comment: Store Blocking Buffer at 4° C prior to opening. DO NOT FREEZE. Expiration date is six (6) months from date of receipt.

Expiry Date: 6 months

Target Details

Background:

Synonyms: Multiplex Blocking Buffer, Immunoblot Blocking Buffer, Blocking Solution, Blocking Buffer Western Blot, Western Blot Blocking Buffer, Alexa Dye Blocking Buffer, DyLight Blocking Buffer, colorimetric, chemiluminescent blocking buffer, WB block, BlockOut, B

Background: BlockOut® is an universal blocking buffer solution for Western Blotting. It is specifically designed for colorimetric, chemiluminescent, and fluorescent western blotting. Universal Blocking Buffer is recommended for blocking when phospho specific antibodi