10X HDAC Assay Buffer

Catalog No. ABIN925590

Supplier Product No.: mb-068-0020

Quick Overview for 10X HDAC Assay Buffer (ABIN925590)

Product Details

Supplier Product No.: mb-068-0020

Supplier: Rockland

Purpose: 10X HDAC Assay Buffer

Purification: This product was aseptically filtered through a 0.22 micron filter into clean, pre-sterilized containers. The product was tested on trypticase soy agar for 24 hours, 48 hours and 72 hours and was found to be negative for bacteria.

Sterility: Sterile filtered

Application Details

Application Notes:

Application Note: This product is a 10X concentrated stock solution.  Prepare a 1X working solution by diluting 1 part 10X concentrate with 9 parts distilled-deionized water or equivalent. Diluted (1X) HDAC Assay buffer consists of 25 mM Tris Cl, 137 mM NaCl, 2.7 mM KCl a

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 10 X

Handling Advice: Some salts may precipitate out of solution at lower temperature. Allow buffer to equilibrate to room temperature

Storage: -20 °C

Storage Comment: Store HDAC Assay Buffer at -20° C or colder prior to opening. Aliquot and re-freeze for best results. Some salts may precipitate out of solution at lower temperature. Allow buffer to equilibrate to room temperature (18° to 26° C) to restore solubility Expiration date is six (6) months from date of receipt.

Expiry Date: 6 months

Target Details

Background:

Synonyms: HDAC Activity Assay Kit

Background: Histone deacetylases (HDACs) are typically found in multiprotein complexes and are tightly regulated by subcellular localization, phosphorylation, and likely by other mechanisms. HDAC assays allow the determination of deacetylase activity and provide acc