HDAC Assay Buffer
EAA
10 X
Quick Overview for HDAC Assay Buffer (ABIN925590)
Application
Enzyme Activity Assay (EAA)
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Supplier Product No.
- mb-068-0020
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Supplier
- Rockland
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Characteristics
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Sterilization: Sterile Aseptic Filter
HDAC Assay Buffer is typically employed with appropriate standards and controls.
Synonyms: HDAC Activity Assay Kit
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Blocking Buffer for Fluorescent Western Blotting
BR, Lbl, WB 1 X
10X TTBS (pH 7.5)BCA, IA 10X
Fluoromount-G®FISH, ICC, IHC (fro), IHC (p), IHC (wm), PLA, TUNEL
10X PBS pH 7.210 X
RIPA Lysis BufferCLy, IP, RIA, WB 1 X
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Application Notes
- This product is a 10X concentrated stock solution. Prepare a 1X working solution by diluting 1 part 10X concentrate with 9 parts distilled-deionized water or equivalent. Diluted (1X) HDAC Assay buffer consists of 25 mM Tris Cl, 137 mM NaCl, 2.7 mM KCl and 1.0 mM MgCl2 at pH 8.0.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 10 X
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Storage
- -20 °C
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Background
- Histone deacetylases (HDACs) are typically found in multiprotein complexes and are tightly regulated by subcellular localization, phosphorylation, and likely by other mechanisms. HDAC assays allow the determination of deacetylase activity and provide accurate activity information that is reflective of endogenous regulation.
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