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Blotto Blocking Reagent Immunoanalytical Grade (Non-Fat Dry Milk)

Analytical Grade BCA, ELISA, FM, IP, WB 1 X
Rockland
Catalog No. ABIN925606
Supplier Product No.: b501-0500

Quick Overview for Blotto Blocking Reagent Immunoanalytical Grade (Non-Fat Dry Milk) (ABIN925606)

Application

Biochemical Assay (BCA), ELISA, Fluorescence Microscopy (FM), Immunoprecipitation (IP), Western Blotting (WB)

Grade

Analytical Grade
  • Supplier Product No.

    b501-0500

    Supplier

    Rockland

    Purpose

    Blotto Immunoanalytical Grade (Non-Fat Dry Milk)

    Purification

    This product contains highly purified immunoanalytical grade non-fat dry milk.
  • Application Notes

    Application Note: Use Rockland's BLOTTO whenever procedures call for immunoanalytical grade non-fat dry milk.   Alternative blocking agents such as BLOTTO A (solution of TBS, 5 % non-fat dry milk and 0.05 % Tween-20) and BLOTTO B (solution of TBS, 1 % non-fat dry milk, 1 % BSA

    Western Blot Dilution: User Optimized

    ELISA Dilution: User Optimized

    IF Microscopy Dilution: User Optimized

    Other: User Optimized

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    Restore with deionized water (or equivalent)

    Concentration

    1 X

    Handling Advice

    After reconstitution use blocking buffers immediately.

    Storage

    4 °C,-20 °C

    Storage Comment

    Store container at room temperature prior to opening. After reconstitution, use blocking buffers immediately. Dilute solutions may be stored at 4° C for up to four (4) days. Solutions containing BLOTTO may be frozen. Expiration date is one (1) year from date of receipt.

    Expiry Date

    12 months
  • Background

    Synonyms: Blotto Immunoanalytical Grade Non-Fat Dry Milk, Blocking reagent for western, antibody dilution buffer

    Background: Rockland Immunochemicals produces the following blocking buffer reagents: BSA, BLOTTO, BLOTTO A, BLOTTO B and Blocking Buffer for Fluorescent Western Blotting (see related products below).  These products are intended to block non-specific binding of prot

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