CCA1 antibody
The Rabbit Polyclonal anti-CCA1 antibody has been validated for WB. It is suitable to detect CCA1 in samples from Arabidopsis thaliana.
Quick Overview for CCA1 antibody (ABIN4966136)
Target
CCA1
(Circadian Clock Associated 1 (CCA1))
Reactivity
Arabidopsis thaliana
Host
Rabbit
Clonality
Polyclonal
Application
Western Blotting (WB)
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Cross-Reactivity (Details)
- Not reactive in: no confirmed exceptions from predicted reactivity are currently known
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Predicted Reactivity
- please inquire
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Immunogen
- KLH-conjugated synthetic peptide derived from Arabidopsis thaliana CCA1 protein sequence, UniProt:P92973, TAIR:AT2G46830
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Application Notes
- 1 : 500 with most sensitive ECL reagent (WB)
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Comment
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Important note about protein extractionTranscription factors are best isolated by freezing nucelar extract in liquid nitrogen after adding the extraction buffer, followed by thawing directly in 100°C heating block not in RT. When the samples were heated for 10 min, spinning of the extract for 5 min is needed to remove the cell debris. From the liquid extract measurement can be done and the rest, frozen in liquid Nitrogen, in aliquots. Frozen samples will not be thawed again to 100°C, but on RT and loaded as soon as they are liquid. Such precautions are necessary to take to be able to detect a transcription factor using antibodies.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- For reconstitution add 50 μL of sterile water.
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Storage
- -20 °C
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Storage Comment
- store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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- CCA1 (Circadian Clock Associated 1 (CCA1))
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Background
- CCA1 (Circadian clocl associated 1) encodes a transcriptional repressor that performs overlapping functions with LHY in a regulatory feedback loop that is closely associated with the circadian oscillator of Arabidopsis. Binds to the evening element in the promoter of TOC1 and represses TOC1 transcription. CCA1 and LHY colocalize in the nucleus and form heterodimers in vivo. CCA1 and LHY function synergistically in regulating circadian rhythms of Arabidopsis.
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Molecular Weight
- 67 | 80 kDa
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UniProt
- P92973
Target
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