Chloroplastic Lipoxygenase antibody (AA 257-450)
The Rabbit Polyclonal anti-Chloroplastic Lipoxygenase antibody has been validated for WB. It is suitable to detect Chloroplastic Lipoxygenase in samples from Arabidopsis thaliana and Grapes.
Catalog No. ABIN190712
Quick Overview for Chloroplastic Lipoxygenase antibody (AA 257-450) (ABIN190712)
Target
Chloroplastic Lipoxygenase (LoxC)
Reactivity
Arabidopsis thaliana, Grapes
Host
Rabbit
Clonality
Polyclonal
Application
Western Blotting (WB)
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Binding Specificity
- AA 257-450
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Cross-Reactivity (Details)
- Not reactive in: no confirmed exceptions from predicted reactivity known in the moment
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Predicted Reactivity
- dicots including: Brassica napus
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Characteristics
- Expected / apparent Molecular Weight of the Antigene: 102 / 97 kDa
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Purification
- serum
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Immunogen
- recombinant Arabidopsis thaliana protein, loop (aa 257-450) P38418, At3g45140
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Application Notes
- 1: 50 000 with alkaline phosphatase (WB)
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Comment
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a weak band at around 84 kDa is detected as a probable result of cross-reaction with another lipoxygenase
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- For reconstitution add 100 μL of sterile water.
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Handling Advice
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Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles. -
Storage
- -20 °C
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Storage Comment
- store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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- Chloroplastic Lipoxygenase (LoxC)
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Alternative Name
- Chloroplastic lipoxygenase (LOX-C)
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Background
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AGI Code: At3g45140
Lipoxygenases (LOXs, EC 1.13.11.12, synonym: lipoxydases) are a family of enzymes that catalyze oxygenation of polyunsaturated fatty acids (PUFAs) into lipidhydroperoxides (LOOHs) involved in responses to stresses. LOXs has been found to play a role in plant growth and development, senescence as well as can be activated in response to environamental stress (drought, heavy metals). Alternative name of the antigen: Lipoxygenase 2 -
Molecular Weight
- expected: 102 kDa, apparent: 97 kDa
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UniProt
- P38418
Target
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