COXIIB antibody
The Rabbit Polyclonal anti-COXIIB antibody has been validated for WB. It is suitable to detect COXIIB in samples from Chlamydomonas reinhardtii. There are 3+ publications available.
(3 references)
Catalog No. ABIN3197486
Quick Overview for COXIIB antibody (ABIN3197486)
Target
COXIIB
(Cytochrome Oxidase Subunit II B (COXIIB))
Reactivity
Chlamydomonas reinhardtii
Host
Rabbit
Clonality
Polyclonal
Application
Western Blotting (WB)
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Cross-Reactivity (Details)
- Not reactive in: no confirmed exceptions from predicted reactivity known in the moment
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Characteristics
- Expected / apparent Molecular Weight of the Antigene: 15 kDa
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Purification
- serum
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Immunogen
- GST fusion with the aminoacids 4-153 of the subunit 2b of the Chlamydomonas reinhardtii cytochrome oxidase, UniProt: Q9AU05
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Application Notes
- 1: 5000 with alkaline phosphatase (WB), 1: 25 000 with standard ECL (WB)
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- For reconstitution add 50 μL of sterile water.
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Handling Advice
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Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles. -
Storage
- -20 °C
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Storage Comment
- store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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: "Proton gradient regulation 5-mediated cyclic electron flow under ATP- or redox-limited conditions: a study of ΔATpase pgr5 and ΔrbcL pgr5 mutants in the green alga Chlamydomonas reinhardtii." in: Plant physiology, Vol. 165, Issue 1, pp. 438-52, (2014) (PubMed).
: "Trophic status of Chlamydomonas reinhardtii influences the impact of iron deficiency on photosynthesis." in: Photosynthesis research, Vol. 105, Issue 1, pp. 39-49, (2010) (PubMed).
: "Target of rapamycin and LST8 proteins associate with membranes from the endoplasmic reticulum in the unicellular green alga Chlamydomonas reinhardtii." in: Eukaryotic cell, Vol. 7, Issue 2, pp. 212-22, (2008) (PubMed).
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: "Proton gradient regulation 5-mediated cyclic electron flow under ATP- or redox-limited conditions: a study of ΔATpase pgr5 and ΔrbcL pgr5 mutants in the green alga Chlamydomonas reinhardtii." in: Plant physiology, Vol. 165, Issue 1, pp. 438-52, (2014) (PubMed).
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- COXIIB (Cytochrome Oxidase Subunit II B (COXIIB))
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Background
- COXII - mitochondrial cytochrome oxidase subunit II is located in inner mitochondrial membrane. The enzyme is a component of the respiratory chain complex involved in the transfer of electrons from cytochrome c to oxygen.
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Molecular Weight
- 15 kDa
Target
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