DCAR/CLEC4B antibody
The Rat Monoclonal anti-DCAR/CLEC4B antibody has been validated for FACS and ELISA. It is suitable to detect DCAR/CLEC4B in samples from Mouse.
Catalog No. ABIN472529
Quick Overview for DCAR/CLEC4B antibody (ABIN472529)
Target
DCAR/CLEC4B (CLEC4B)
(C-Type Lectin Domain Family 4, Member B (CLEC4B))
Reactivity
Mouse
Host
Rat
Clonality
Monoclonal
Application
Flow Cytometry (FACS), ELISA
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Specificity
- Detects rmDCAR in direct ELISAs.
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Purification
- Protein G purified
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Sterility
- 0.2 μm filtered
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Immunogen
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NS0-derived rmDCAR.
Type of Immunogen: Recombinant protein -
Isotype
- IgG2a
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Application Notes
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Approved: ELISA, Flo
Usage: Flow Cytometry: This antibody was validated for flow cytometry using dendritic cells. Dilute this antibody to 25 μg/mL and add 10 μL of the diluted solution to 1-2.5 x 10^5 dells in a total reaction volume not exceeding 200 L. The binding of unlabeled monoclonal antibodies may be visualized by adding 10 μL of a 25 μg/mL stock solution of a secondary developing reagent such as goat anti-rat IgG conjugated to a fluorochrome. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect mouse DCAR. The detection limit for rmDCAR is approximately 5 ng/well. -
Comment
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Target Species of Antibody: Mouse
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Reconstitute, sterile PBS, 40-50 % glycerol. If 0.2 mL is used, the concentration will be 0.5 mg/mL.
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Concentration
- Lot specific
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Buffer
- Lyophilized powder from a 0.2 um filtered solution in PBS, 5 % trehalose. Reconstitute, sterile PBS, 40-50 % glycerol. If 0.2 mL is used, the concentration will be 0.5 mg/mL.
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- DCAR/CLEC4B (CLEC4B) (C-Type Lectin Domain Family 4, Member B (CLEC4B))
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Alternative Name
- CLEC4B
Target
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