EUG1 Protein (AA 30-517) (His tag)
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- Target
- EUG1 (Protein disulfide-isomerase EUG1 (EUG1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 30-517
- Origin
- Saccharomyces cerevisiae
- Source
- Yeast
- Purification tag / Conjugate
- This EUG1 protein is labelled with His tag.
- Application
- ELISA
- Sequence
- T PGSDLLVLTE KKFKSFIESH PLVLVEFFAP WCLHSQILRP HLEEAASILK EHNVPVVQID CEANSMVCLQ QTINTYPTLK IFKNGRIFDG QVYRGVKITD EITQYMIQLY EASVIYLNSE DEIQPYLENA TLPVVINRGL TGLNETYQEV ALDLAEDYVF LSLLDSEDKS LSIHLPNTTE PILFDGNVDS LVGNSVALTQ WLKVVILPYF TDIEPDLFPK YISSNLPLAY FFYTSEEELE DYTDLFTQLG KENRGQINFI ALNSTMFPHH VRFLNMREQF PLFAIHNMIN NLKYGLPQLP EEEYAKLEKP QPLDRDMIVQ LVKDYREGTA KPIVKSEEIP KEQKSNVYKI VGKTHDDIVH DDDKDVLVKY YATWCIHSKR FAPIYEEIAN VLASDESVRD KILIAEVDSG ANDILSFPVT GYPTIALYPA GNNSKPIIFN KIRNLEDVFE FIKESGTHHI DGQAIYDKLH QAKDSEVSTE DTVHDEL
- Specificity
- Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Bakers yeast)
- Characteristics
- Please inquire if you are interested in this recombinant protein expressed in E. coli, mammalien cells or by baculovirus infection. Be aware about differences in price and lead time.
- Purity
- > 90 %
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- Comment
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The yeast protein expression system is the most economical and efficient eukaryotic system for secretion and intracellular expression. A protein expressed by the mammalian cell system is of very high-quality and close to the natural protein. But the low expression level, the high cost of medium and the culture conditions restrict the promotion of mammalian cell expression systems. The yeast protein expression system serve as a eukaryotic system integrate the advantages of the mammalian cell expression system. A protein expressed by yeast system could be modificated such as glycosylation, acylation, phosphorylation and so on to ensure the native protein conformation. It can be used to produce protein material with high added value that is very close to the natural protein. Our proteins produced by yeast expression system has been used as raw materials for downstream preparation of monoclonal antibodies.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Concentration
- 0.2-2 mg/mL
- Buffer
- Tris-based buffer, 50 % glycerol
- Handling Advice
- Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week
- Storage
- -20 °C
- Storage Comment
- Store at -20 °C, for extended storage, conserve at -20 °C or -80 °C.
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- Target
- EUG1 (Protein disulfide-isomerase EUG1 (EUG1))
- Background
-
Recommended name: Protein disulfide-isomerase EUG1.
Short name= PDI.
EC= 5.3.4.1.
Alternative name(s): Endoplasmic reticulum protein EUG1 - UniProt
- P32474
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